Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia
Introduction. Cytomegalovirus (CMV) is a DNA-containing virus that is widespread worldwide and is of great importance in infectious pathology of children and adults. The aim of this study is to evaluate the prevalence of CMV among children and immunocompromised patients in the Nizhny Novgorod reg...
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Central Research Institute for Epidemiology
2024-12-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/16696/949 |
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| author | Ol’ga E. Van’kova Nina F. Brusnigina Nadezhda A. Novikova |
| author_facet | Ol’ga E. Van’kova Nina F. Brusnigina Nadezhda A. Novikova |
| author_sort | Ol’ga E. Van’kova |
| collection | DOAJ |
| description | Introduction. Cytomegalovirus (CMV) is a DNA-containing virus that is widespread worldwide and is of great importance in infectious pathology of children and adults.
The aim of this study is to evaluate the prevalence of CMV among children and immunocompromised patients in the Nizhny Novgorod region (central Russia) and to perform a phylogenetic analysis of the identified strains.
Materials and methods. DNA samples of CMV detected in frequently ill children and adult recipients of solid organs were studied. The genetic diversity of CMV was assessed for two variable genes: UL55(gB) and UL73(gN), using NGS technology on the Illumina platform. Phylogenetic trees were constructed in the MEGA X program, the reliability of the cluster topology on the trees was confirmed using the rapid bootstrap method based on the generation of 500 pseudo-replicas.
Results. Circulation of 5 CMV genotypes by gene UL55(gB) and 5 genotypes by gene UL73(gN) was established in the territory of the Nizhny Novgorod region. While genotypes gB1 and gB2 dominated both in children and in adults, genotype gB5 was detected only in children. The spectrum of gN genotypes was more diverse: genotypes gN4a and gN3b prevailed in children, and gN1 and gN4b genotypes were predominant in adults. The obtained results allowed us to establish the similarity of the landscape of CMV genotypes circulating in Russia (Nizhny Novgorod region), Brazil, China and the USA.
Conclusion. The obtained data indicate the similarity of the landscape of CMV genotypes circulating in Russia (Nizhny Novgorod region), Brazil, China and the USA: in children, the predominant genotypes are gB1 (40.0%), gB2 (33.3%), gN4a (42.8%), and gN3b (28.6%), while in adults (recipients of solid organs) genotypes gB1 (37.5%), gB2 (37.5%), gN1 (26.3%), and gN4b (26.3%) are prevailed. |
| format | Article |
| id | doaj-art-9218b27c969d48c284775f2415e4b4ab |
| institution | Kabale University |
| issn | 0507-4088 2411-2097 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj-art-9218b27c969d48c284775f2415e4b4ab2025-01-22T15:18:43ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972024-12-0169653554510.36233/0507-4088-2771257Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central RussiaOl’ga E. Van’kova0https://orcid.org/0000-0002-9838-1133Nina F. Brusnigina1https://orcid.org/0000-0003-4582-5623Nadezhda A. Novikova2https://orcid.org/0000-0002-3710-6648Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. BlokhinaNizhny Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. BlokhinaNizhny Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. BlokhinaIntroduction. Cytomegalovirus (CMV) is a DNA-containing virus that is widespread worldwide and is of great importance in infectious pathology of children and adults. The aim of this study is to evaluate the prevalence of CMV among children and immunocompromised patients in the Nizhny Novgorod region (central Russia) and to perform a phylogenetic analysis of the identified strains. Materials and methods. DNA samples of CMV detected in frequently ill children and adult recipients of solid organs were studied. The genetic diversity of CMV was assessed for two variable genes: UL55(gB) and UL73(gN), using NGS technology on the Illumina platform. Phylogenetic trees were constructed in the MEGA X program, the reliability of the cluster topology on the trees was confirmed using the rapid bootstrap method based on the generation of 500 pseudo-replicas. Results. Circulation of 5 CMV genotypes by gene UL55(gB) and 5 genotypes by gene UL73(gN) was established in the territory of the Nizhny Novgorod region. While genotypes gB1 and gB2 dominated both in children and in adults, genotype gB5 was detected only in children. The spectrum of gN genotypes was more diverse: genotypes gN4a and gN3b prevailed in children, and gN1 and gN4b genotypes were predominant in adults. The obtained results allowed us to establish the similarity of the landscape of CMV genotypes circulating in Russia (Nizhny Novgorod region), Brazil, China and the USA. Conclusion. The obtained data indicate the similarity of the landscape of CMV genotypes circulating in Russia (Nizhny Novgorod region), Brazil, China and the USA: in children, the predominant genotypes are gB1 (40.0%), gB2 (33.3%), gN4a (42.8%), and gN3b (28.6%), while in adults (recipients of solid organs) genotypes gB1 (37.5%), gB2 (37.5%), gN1 (26.3%), and gN4b (26.3%) are prevailed.https://virusjour.crie.ru/jour/article/viewFile/16696/949сytomegalovirusgenotypephylogenetic analysisgenetic diversity |
| spellingShingle | Ol’ga E. Van’kova Nina F. Brusnigina Nadezhda A. Novikova Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia Вопросы вирусологии сytomegalovirus genotype phylogenetic analysis genetic diversity |
| title | Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia |
| title_full | Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia |
| title_fullStr | Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia |
| title_full_unstemmed | Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia |
| title_short | Prevalence and phylogenetic analysis of cytomegalovirus (Orthoherpesviridae: <i>Cytomegalovirus: Cytomegalovirus humanbeta5</i>) genetic variants from children and immunocompromised patients in central Russia |
| title_sort | prevalence and phylogenetic analysis of cytomegalovirus orthoherpesviridae i cytomegalovirus cytomegalovirus humanbeta5 i genetic variants from children and immunocompromised patients in central russia |
| topic | сytomegalovirus genotype phylogenetic analysis genetic diversity |
| url | https://virusjour.crie.ru/jour/article/viewFile/16696/949 |
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