Significance of Algal Polymer in Designing Amphotericin B Nanoparticles
Development of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have develop...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/564573 |
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| author | Saurabh Bhatia Vikash Kumar Kiran Sharma Kalpana Nagpal Tanmoy Bera |
| author_facet | Saurabh Bhatia Vikash Kumar Kiran Sharma Kalpana Nagpal Tanmoy Bera |
| author_sort | Saurabh Bhatia |
| collection | DOAJ |
| description | Development of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have developed a novel carrier system in form of polyelectrolyte complex of AmB by using chitosan (CS) and porphyran (POR) as two oppositely charged polymers with TPP as a crosslinking agent. Initially POR was isolated from Porphyra vietnamensis followed by the fact that its alkali induced safe reduction in molecular weight was achieved. Formulation was optimized using three-factor three-level (33) central composite design. High concentration of POR in NPs was confirmed by sulfated polysaccharide (SP) assay. Degradation and dissolution studies suggested the stability of NPs over wide pH range. Hemolytic toxicity data suggested the safety of prepared formulation. In vivo and in vitro antifungal activity demonstrated the high antifungal potential of optimized formulation when compared with standard drug and marketed formulations. Throughout the study TPP addition did not cause any significant changes. Therefore, these experimental oral NPs may represent an interesting carrier system for the delivery of AmB. |
| format | Article |
| id | doaj-art-920e31f10fb742db8264d475fd08e1eb |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-920e31f10fb742db8264d475fd08e1eb2025-02-03T01:31:03ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/564573564573Significance of Algal Polymer in Designing Amphotericin B NanoparticlesSaurabh Bhatia0Vikash Kumar1Kiran Sharma2Kalpana Nagpal3Tanmoy Bera4PDMCOP, Bahadurgarh, Haryana 124507, IndiaPDMCOP, Bahadurgarh, Haryana 124507, IndiaDepartment of Pharmaceutical Sciences, Jamia Hamdard, New Delhi 110062, IndiaSchool of Pharmacy, Faculty of Applied Medical Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, IndiaDevelopment of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have developed a novel carrier system in form of polyelectrolyte complex of AmB by using chitosan (CS) and porphyran (POR) as two oppositely charged polymers with TPP as a crosslinking agent. Initially POR was isolated from Porphyra vietnamensis followed by the fact that its alkali induced safe reduction in molecular weight was achieved. Formulation was optimized using three-factor three-level (33) central composite design. High concentration of POR in NPs was confirmed by sulfated polysaccharide (SP) assay. Degradation and dissolution studies suggested the stability of NPs over wide pH range. Hemolytic toxicity data suggested the safety of prepared formulation. In vivo and in vitro antifungal activity demonstrated the high antifungal potential of optimized formulation when compared with standard drug and marketed formulations. Throughout the study TPP addition did not cause any significant changes. Therefore, these experimental oral NPs may represent an interesting carrier system for the delivery of AmB.http://dx.doi.org/10.1155/2014/564573 |
| spellingShingle | Saurabh Bhatia Vikash Kumar Kiran Sharma Kalpana Nagpal Tanmoy Bera Significance of Algal Polymer in Designing Amphotericin B Nanoparticles The Scientific World Journal |
| title | Significance of Algal Polymer in Designing Amphotericin B Nanoparticles |
| title_full | Significance of Algal Polymer in Designing Amphotericin B Nanoparticles |
| title_fullStr | Significance of Algal Polymer in Designing Amphotericin B Nanoparticles |
| title_full_unstemmed | Significance of Algal Polymer in Designing Amphotericin B Nanoparticles |
| title_short | Significance of Algal Polymer in Designing Amphotericin B Nanoparticles |
| title_sort | significance of algal polymer in designing amphotericin b nanoparticles |
| url | http://dx.doi.org/10.1155/2014/564573 |
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