Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry
The aim of this work was to develop an automated on-line solid phase extraction (SPE) with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples. Samples were extracted with 0.2% formic acid acetonitrile solution, purified by on-line...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2020-12-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301415 |
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| author | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li |
| author_facet | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li |
| author_sort | Junmei Ma |
| collection | DOAJ |
| description | The aim of this work was to develop an automated on-line solid phase extraction (SPE) with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples. Samples were extracted with 0.2% formic acid acetonitrile solution, purified by on-line SPE device with HLB column, then separated by XBridge C18 column, using 0.1% formic acid solution and acetonitrile as the mobile phase. Mass spectrometric data was acquired under multiple reaction monitoring (MRM) mode using positive ionization electrospray. Internal standard method was used in the quantification, good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990. The limits of detection were in the range of 0.125–2.00 μg/kg and the limits of quantitation were in the range of 0.250–5.00 μg/kg. Recoveries of the method were in range of 78.3%–99.3%, relative standard deviation were lower than 10%. The method was simple, sensitivity, and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish. |
| format | Article |
| id | doaj-art-920d904a853b438c965a2992d529701e |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-920d904a853b438c965a2992d529701e2025-02-03T02:38:53ZengTsinghua University PressFood Science and Human Wellness2213-45302020-12-0194363369Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometryJunmei Ma0Sufang Fan1Lei Sun2Liangna He3Yan Zhang4Qiang Li5Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, ChinaHebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, ChinaHebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, China; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, China; Corresponding authors.Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 050227, Shijiazhuang, China; Corresponding authors.The aim of this work was to develop an automated on-line solid phase extraction (SPE) with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples. Samples were extracted with 0.2% formic acid acetonitrile solution, purified by on-line SPE device with HLB column, then separated by XBridge C18 column, using 0.1% formic acid solution and acetonitrile as the mobile phase. Mass spectrometric data was acquired under multiple reaction monitoring (MRM) mode using positive ionization electrospray. Internal standard method was used in the quantification, good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990. The limits of detection were in the range of 0.125–2.00 μg/kg and the limits of quantitation were in the range of 0.250–5.00 μg/kg. Recoveries of the method were in range of 78.3%–99.3%, relative standard deviation were lower than 10%. The method was simple, sensitivity, and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish.http://www.sciencedirect.com/science/article/pii/S2213453020301415Liquid chromatography–tandem mass spectrometryOn-line solid phase extractionSulfonamidesInternal standard quantification |
| spellingShingle | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry Food Science and Human Wellness Liquid chromatography–tandem mass spectrometry On-line solid phase extraction Sulfonamides Internal standard quantification |
| title | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry |
| title_full | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry |
| title_fullStr | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry |
| title_full_unstemmed | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry |
| title_short | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry |
| title_sort | rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on line solid phase extraction coupled to liquid chromatography tandem mass spectrometry |
| topic | Liquid chromatography–tandem mass spectrometry On-line solid phase extraction Sulfonamides Internal standard quantification |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301415 |
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