Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients
Oxidative stress in the lung is important in the pathogenesis of COPD. Published data indicate that glucocorticoids inhibit blood cells in their capacity to produce reactive oxygen species (ROS). We investigated the effect of Fluticasone propionate (FP) on the ROS production capabilities of pulmonar...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/096293500411578 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554235315617792 |
|---|---|
| author | Gert T. Verhoeven Annemarie J.M. Wijkhuijs Herbert Hooijkaas Henk C. Hoogsteden Wim Sluiter |
| author_facet | Gert T. Verhoeven Annemarie J.M. Wijkhuijs Herbert Hooijkaas Henk C. Hoogsteden Wim Sluiter |
| author_sort | Gert T. Verhoeven |
| collection | DOAJ |
| description | Oxidative stress in the lung is important in the pathogenesis of COPD. Published data indicate that glucocorticoids inhibit blood cells in their capacity to produce reactive oxygen species (ROS). We investigated the effect of Fluticasone propionate (FP) on the ROS production capabilities of pulmonary cells. Bronchoalveolar lavage (BAL) was performed in smoking COPD patients, before and after a six month, placebo-controlled treatment with FP. BAL cells were stimulated with phorbol myristrate acetate (PMA) alone, and together with superoxide dismutase (SOD). From kinetic plots of ferricytochrome-c conversion we calculated the maximal rate of superoxide production: Vmax. We also examined BAL cell subsets and performed correlation analyses on ROS production and relevant clinical determinants. Paired results were obtained from 6 FP- and 9 placebo-treated patients. No significant change of Vmax was found in both patient groups. Also BAL cellularity was unchanged. Correlation analyses showed a significant (inverse) association
of Vmax with the number of cigarettes smoked per day. We concluded that a potent inhaled glucocorticoid had no effect on the ROS production capability of BAL cells from smoking COPD patients. Apparently, heavy smoking impaired the ability of alveolar macrophages to produce ROS, which was not further decreased by FP. |
| format | Article |
| id | doaj-art-920d7cd9d0394453b53cc5af8f349034 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-920d7cd9d0394453b53cc5af8f3490342025-02-03T05:51:59ZengWileyMediators of Inflammation0962-93511466-18612000-01-019210911310.1080/096293500411578Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patientsGert T. Verhoeven0Annemarie J.M. Wijkhuijs1Herbert Hooijkaas2Henk C. Hoogsteden3Wim Sluiter4Department of Pulmonary and Intensive Care Medicine, Erasmus University Medical Center Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University Medical Center Rotterdam, The NetherlandsDepartment of Immunology, Erasmus University Medical Center Rotterdam, The NetherlandsDepartment of Pulmonary and Intensive Care Medicine, Erasmus University Medical Center Rotterdam, The NetherlandsDepartment of Biochemistry, Erasmus University Medical Center Rotterdam, The NetherlandsOxidative stress in the lung is important in the pathogenesis of COPD. Published data indicate that glucocorticoids inhibit blood cells in their capacity to produce reactive oxygen species (ROS). We investigated the effect of Fluticasone propionate (FP) on the ROS production capabilities of pulmonary cells. Bronchoalveolar lavage (BAL) was performed in smoking COPD patients, before and after a six month, placebo-controlled treatment with FP. BAL cells were stimulated with phorbol myristrate acetate (PMA) alone, and together with superoxide dismutase (SOD). From kinetic plots of ferricytochrome-c conversion we calculated the maximal rate of superoxide production: Vmax. We also examined BAL cell subsets and performed correlation analyses on ROS production and relevant clinical determinants. Paired results were obtained from 6 FP- and 9 placebo-treated patients. No significant change of Vmax was found in both patient groups. Also BAL cellularity was unchanged. Correlation analyses showed a significant (inverse) association of Vmax with the number of cigarettes smoked per day. We concluded that a potent inhaled glucocorticoid had no effect on the ROS production capability of BAL cells from smoking COPD patients. Apparently, heavy smoking impaired the ability of alveolar macrophages to produce ROS, which was not further decreased by FP.http://dx.doi.org/10.1080/096293500411578 |
| spellingShingle | Gert T. Verhoeven Annemarie J.M. Wijkhuijs Herbert Hooijkaas Henk C. Hoogsteden Wim Sluiter Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients Mediators of Inflammation |
| title | Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients |
| title_full | Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients |
| title_fullStr | Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients |
| title_full_unstemmed | Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients |
| title_short | Effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking COPD patients |
| title_sort | effect of an inhaled glucocorticoid on reactive oxygen species production by bronchoalveolar lavage cells from smoking copd patients |
| url | http://dx.doi.org/10.1080/096293500411578 |
| work_keys_str_mv | AT gerttverhoeven effectofaninhaledglucocorticoidonreactiveoxygenspeciesproductionbybronchoalveolarlavagecellsfromsmokingcopdpatients AT annemariejmwijkhuijs effectofaninhaledglucocorticoidonreactiveoxygenspeciesproductionbybronchoalveolarlavagecellsfromsmokingcopdpatients AT herberthooijkaas effectofaninhaledglucocorticoidonreactiveoxygenspeciesproductionbybronchoalveolarlavagecellsfromsmokingcopdpatients AT henkchoogsteden effectofaninhaledglucocorticoidonreactiveoxygenspeciesproductionbybronchoalveolarlavagecellsfromsmokingcopdpatients AT wimsluiter effectofaninhaledglucocorticoidonreactiveoxygenspeciesproductionbybronchoalveolarlavagecellsfromsmokingcopdpatients |