Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-d...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/106743 |
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| author | Małgorzata Wiese Andrzej Eljaszewicz Anna Helmin-Basa Marek Andryszczyk Ilona Motyl Jolanta Wieczyńska Lidia Gackowska Izabela Kubiszewska Milena Januszewska Jacek Michałkiewicz |
| author_facet | Małgorzata Wiese Andrzej Eljaszewicz Anna Helmin-Basa Marek Andryszczyk Ilona Motyl Jolanta Wieczyńska Lidia Gackowska Izabela Kubiszewska Milena Januszewska Jacek Michałkiewicz |
| author_sort | Małgorzata Wiese |
| collection | DOAJ |
| description | The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties. |
| format | Article |
| id | doaj-art-91fcc715835e44c49122f6f15f8c4839 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-91fcc715835e44c49122f6f15f8c48392025-02-03T05:50:53ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/106743106743Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic CellsMałgorzata Wiese0Andrzej Eljaszewicz1Anna Helmin-Basa2Marek Andryszczyk3Ilona Motyl4Jolanta Wieczyńska5Lidia Gackowska6Izabela Kubiszewska7Milena Januszewska8Jacek Michałkiewicz9Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandCollegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandCollegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandFaculty of Mechanical Engineering, University of Technology and Sciences in Bydgoszcz, Kaliskiego 7 Street, 85-789 Bydgoszcz, PolandThe Institute of Technology Fermentation and Microbiology, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Wólczańska 171/173 Street, 90-924 Łódź, PolandDepartment of Clinical Microbiology and Immunology, Children’s Memorial Hospital, Aleja Dzieci Polskich 20, 04-730 Warsaw, PolandCollegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandCollegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandCollegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, PolandDepartment of Clinical Microbiology and Immunology, Children’s Memorial Hospital, Aleja Dzieci Polskich 20, 04-730 Warsaw, PolandThe aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.http://dx.doi.org/10.1155/2015/106743 |
| spellingShingle | Małgorzata Wiese Andrzej Eljaszewicz Anna Helmin-Basa Marek Andryszczyk Ilona Motyl Jolanta Wieczyńska Lidia Gackowska Izabela Kubiszewska Milena Januszewska Jacek Michałkiewicz Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells Journal of Immunology Research |
| title | Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells |
| title_full | Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells |
| title_fullStr | Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells |
| title_full_unstemmed | Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells |
| title_short | Lactic Acid Bacteria Strains Exert Immunostimulatory Effect on H. pylori-Induced Dendritic Cells |
| title_sort | lactic acid bacteria strains exert immunostimulatory effect on h pylori induced dendritic cells |
| url | http://dx.doi.org/10.1155/2015/106743 |
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