Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds
Lipoxygenase (EC 1.13.11.12) was partially purified from germinated seeds of Cucumeropsis manii to a purification fold of 47.14, enzyme activity recovery of 72.18%, and specific activity of 326.25 units/mg protein, using a three-step process of centrifugation, ammonium sulphate precipitation and gel...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/864095 |
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| author | Sabinus O. O. Eze Bennett C. Nwanguma |
| author_facet | Sabinus O. O. Eze Bennett C. Nwanguma |
| author_sort | Sabinus O. O. Eze |
| collection | DOAJ |
| description | Lipoxygenase (EC 1.13.11.12) was partially purified from germinated seeds of Cucumeropsis manii to a purification fold of 47.14, enzyme activity recovery of 72.18%, and specific activity of 326.25 units/mg protein, using a three-step process of centrifugation, ammonium sulphate precipitation and gel filtration. Kinetic properties show maximum activity at pH 6.0 and at optimum temperature of 40°C. Inhibitory effects of the extract from Gongronema latifolium and two other known antioxidants: ascorbic acid and propyl gallate on lipoxygenase from Cucumeropsis manii were studied. Result shows presence of inhibition with IC50 of 4.2×10−3 ± 0.09×10−3 g/L, 4.3×10−2 ± 0.11×10−2 g/L and 7.9×10−2 ± 0.11×10−2 g/L for the extract from ascorbic acid and propyl gallate, respectively. The extract when compared to the other antioxidants exhibits a competitive mechanism of inhibition. This tannin extract could be included during food processing as preservative against food deterioration that might be caused by oxidative enzymes such as lipoxygenase. |
| format | Article |
| id | doaj-art-91f3829bf7d846a2aeafb831e3e3e58a |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-91f3829bf7d846a2aeafb831e3e3e58a2025-02-03T01:29:59ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/864095864095Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii SeedsSabinus O. O. Eze0Bennett C. Nwanguma1Department of Biochemistry, University of Nigeria, Nsukka 410001, NigeriaDepartment of Biochemistry, University of Nigeria, Nsukka 410001, NigeriaLipoxygenase (EC 1.13.11.12) was partially purified from germinated seeds of Cucumeropsis manii to a purification fold of 47.14, enzyme activity recovery of 72.18%, and specific activity of 326.25 units/mg protein, using a three-step process of centrifugation, ammonium sulphate precipitation and gel filtration. Kinetic properties show maximum activity at pH 6.0 and at optimum temperature of 40°C. Inhibitory effects of the extract from Gongronema latifolium and two other known antioxidants: ascorbic acid and propyl gallate on lipoxygenase from Cucumeropsis manii were studied. Result shows presence of inhibition with IC50 of 4.2×10−3 ± 0.09×10−3 g/L, 4.3×10−2 ± 0.11×10−2 g/L and 7.9×10−2 ± 0.11×10−2 g/L for the extract from ascorbic acid and propyl gallate, respectively. The extract when compared to the other antioxidants exhibits a competitive mechanism of inhibition. This tannin extract could be included during food processing as preservative against food deterioration that might be caused by oxidative enzymes such as lipoxygenase.http://dx.doi.org/10.1155/2013/864095 |
| spellingShingle | Sabinus O. O. Eze Bennett C. Nwanguma Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds Journal of Chemistry |
| title | Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds |
| title_full | Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds |
| title_fullStr | Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds |
| title_full_unstemmed | Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds |
| title_short | Effects of Tannin Extract from Gongronema latifolium Leaves on Lipoxygenase Cucumeropsis manii Seeds |
| title_sort | effects of tannin extract from gongronema latifolium leaves on lipoxygenase cucumeropsis manii seeds |
| url | http://dx.doi.org/10.1155/2013/864095 |
| work_keys_str_mv | AT sabinusooeze effectsoftanninextractfromgongronemalatifoliumleavesonlipoxygenasecucumeropsismaniiseeds AT bennettcnwanguma effectsoftanninextractfromgongronemalatifoliumleavesonlipoxygenasecucumeropsismaniiseeds |