The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced inf...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/454176 |
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| author | Julia Romanova Maria Filimonova |
| author_facet | Julia Romanova Maria Filimonova |
| author_sort | Julia Romanova |
| collection | DOAJ |
| description | Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease. |
| format | Article |
| id | doaj-art-91e25511d74e4769b4a5669d265ab87b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-91e25511d74e4769b4a5669d265ab87b2025-02-03T05:43:45ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/454176454176The Effects of Addition of Mononucleotides on Sma nuc Endonuclease ActivityJulia Romanova0Maria Filimonova1Department of Microbiology, Kazan (Volga Region) Federal University, Kremliovskaya Street 18, Kazan 420008, Tatarstan, RussiaDepartment of Microbiology, Kazan (Volga Region) Federal University, Kremliovskaya Street 18, Kazan 420008, Tatarstan, RussiaExamination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease.http://dx.doi.org/10.1100/2012/454176 |
| spellingShingle | Julia Romanova Maria Filimonova The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity The Scientific World Journal |
| title | The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity |
| title_full | The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity |
| title_fullStr | The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity |
| title_full_unstemmed | The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity |
| title_short | The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity |
| title_sort | effects of addition of mononucleotides on sma nuc endonuclease activity |
| url | http://dx.doi.org/10.1100/2012/454176 |
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