Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease
Modern technological lifestyles promote allergic diseases, especially food allergies. The underlying molecular mechanisms remain to be uncovered. Protein acetylation is one of the most important post-translational modifications, and it is involved in regulating multiple body metabolic processes. Thi...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002063 |
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| author | Yanan Sun Ning Liu Huihui Wang Taiqi Qu Fazheng Ren Yixuan Li |
| author_facet | Yanan Sun Ning Liu Huihui Wang Taiqi Qu Fazheng Ren Yixuan Li |
| author_sort | Yanan Sun |
| collection | DOAJ |
| description | Modern technological lifestyles promote allergic diseases, especially food allergies. The underlying molecular mechanisms remain to be uncovered. Protein acetylation is one of the most important post-translational modifications, and it is involved in regulating multiple body metabolic processes. This study aimed to clarify the effects of a high-fat diet (HFD) on allergy risk and the underlying mechanisms. Four-week-old male C57BL/6J mice were randomly divided into two groups and fed a normal fat diet (NFD) or HFD for 24 weeks. Then, serum lipids were measured, and skeletal muscle was collected for acetylome analysis. Compared with the findings in the NFD group, HFD-fed mice were obese and hyperlipidemic. Acetylome analysis also revealed 32 differentially expressed proteins between the HFD and NFD groups. Among these, eight acetylated proteins were upregulated in the HFD group. In addition, 13 and 11 proteins were acetylated only in the HFD group and NFD group, respectively. These proteins were mainly involved in regulating energy metabolism and mitochondrial function. This study provides information regarding the underlying molecular mechanisms by which HFD promotes allergy. |
| format | Article |
| id | doaj-art-91d8506e5f52409790155b9d939dcf02 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-91d8506e5f52409790155b9d939dcf022025-02-03T10:34:08ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123889894Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic diseaseYanan Sun0Ning Liu1Huihui Wang2Taiqi Qu3Fazheng Ren4Yixuan Li5Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China; Corresponding author at: Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.Modern technological lifestyles promote allergic diseases, especially food allergies. The underlying molecular mechanisms remain to be uncovered. Protein acetylation is one of the most important post-translational modifications, and it is involved in regulating multiple body metabolic processes. This study aimed to clarify the effects of a high-fat diet (HFD) on allergy risk and the underlying mechanisms. Four-week-old male C57BL/6J mice were randomly divided into two groups and fed a normal fat diet (NFD) or HFD for 24 weeks. Then, serum lipids were measured, and skeletal muscle was collected for acetylome analysis. Compared with the findings in the NFD group, HFD-fed mice were obese and hyperlipidemic. Acetylome analysis also revealed 32 differentially expressed proteins between the HFD and NFD groups. Among these, eight acetylated proteins were upregulated in the HFD group. In addition, 13 and 11 proteins were acetylated only in the HFD group and NFD group, respectively. These proteins were mainly involved in regulating energy metabolism and mitochondrial function. This study provides information regarding the underlying molecular mechanisms by which HFD promotes allergy.http://www.sciencedirect.com/science/article/pii/S2213453022002063Allergic diseaseHigh fat dietSkeletal muscleAcetylome |
| spellingShingle | Yanan Sun Ning Liu Huihui Wang Taiqi Qu Fazheng Ren Yixuan Li Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease Food Science and Human Wellness Allergic disease High fat diet Skeletal muscle Acetylome |
| title | Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| title_full | Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| title_fullStr | Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| title_full_unstemmed | Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| title_short | Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| title_sort | comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease |
| topic | Allergic disease High fat diet Skeletal muscle Acetylome |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002063 |
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