A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats
Today, there are over 2,000 different biomaterials used for various medical applications, but none of these biomaterials are 100% compatible with all human beings. Coconut fiber is widely available but has not been tested as a safe natural alternative for sutures. Immature coconut fiber is nonabsorb...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2021/8122989 |
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| author | Raghu Babu Pothireddy Angeline Julius Manu Thomas Mathai Ganesh Lakshmanan Beimnet Asfaw Hailemariam |
| author_facet | Raghu Babu Pothireddy Angeline Julius Manu Thomas Mathai Ganesh Lakshmanan Beimnet Asfaw Hailemariam |
| author_sort | Raghu Babu Pothireddy |
| collection | DOAJ |
| description | Today, there are over 2,000 different biomaterials used for various medical applications, but none of these biomaterials are 100% compatible with all human beings. Coconut fiber is widely available but has not been tested as a safe natural alternative for sutures. Immature coconut fiber is nonabsorbable and is effective for cuts and open wounds when used in combination with dehydrated human amnion membrane (dHAM). Immature coconut fiber, tamarind seed polysaccharide (TSP), and dHAM were prepared to test their combinational effect on wound healing in rats. TSP enhanced cell viability, proliferation, and migration in human skin cells and cured wounds both individually and in combination with dHAM. An antibiotic-free combination of the human amniotic membrane with intact epithelium, tamarind seed polysaccharide, and immature coconut fiber provided faster wound healing. Significantly higher wound healing was seen on the 11th day based on an initial 10 mm biopsy punch surgery in Wistar rats compared to control groups. Histological studies revealed thickened dermis edges with more neutrophil infiltration. Collagen deposition in the dermis was homogeneous across the excised skin tissue in the test group, again attesting to the utility of this procedure. This research signifies the use of TSP gel together with the amnion membrane representing a “smart patch” with wound healing potential, which would encourage further research on the smart patch made using a combination of plant and animal biological materials. |
| format | Article |
| id | doaj-art-91cf6fc5efb244169c71e0418b724107 |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-91cf6fc5efb244169c71e0418b7241072025-02-03T01:27:23ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422021-01-01202110.1155/2021/81229898122989A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in RatsRaghu Babu Pothireddy0Angeline Julius1Manu Thomas Mathai2Ganesh Lakshmanan3Beimnet Asfaw Hailemariam4Department of Zoology, Madras Christian College, Affiliated to University of Madras, Chennai 600059, Tamil Nadu, IndiaCentre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai 600126, Tamil Nadu, IndiaDepartment of Zoology, Madras Christian College, Affiliated to University of Madras, Chennai 600059, Tamil Nadu, IndiaDepartment of Anatomy, Asan Memorial Dental College and Hospital, Chennai 603105, Tamil Nadu, IndiaInstitute of Biotechnology, Addis Ababa University, Addis Ababa, EthiopiaToday, there are over 2,000 different biomaterials used for various medical applications, but none of these biomaterials are 100% compatible with all human beings. Coconut fiber is widely available but has not been tested as a safe natural alternative for sutures. Immature coconut fiber is nonabsorbable and is effective for cuts and open wounds when used in combination with dehydrated human amnion membrane (dHAM). Immature coconut fiber, tamarind seed polysaccharide (TSP), and dHAM were prepared to test their combinational effect on wound healing in rats. TSP enhanced cell viability, proliferation, and migration in human skin cells and cured wounds both individually and in combination with dHAM. An antibiotic-free combination of the human amniotic membrane with intact epithelium, tamarind seed polysaccharide, and immature coconut fiber provided faster wound healing. Significantly higher wound healing was seen on the 11th day based on an initial 10 mm biopsy punch surgery in Wistar rats compared to control groups. Histological studies revealed thickened dermis edges with more neutrophil infiltration. Collagen deposition in the dermis was homogeneous across the excised skin tissue in the test group, again attesting to the utility of this procedure. This research signifies the use of TSP gel together with the amnion membrane representing a “smart patch” with wound healing potential, which would encourage further research on the smart patch made using a combination of plant and animal biological materials.http://dx.doi.org/10.1155/2021/8122989 |
| spellingShingle | Raghu Babu Pothireddy Angeline Julius Manu Thomas Mathai Ganesh Lakshmanan Beimnet Asfaw Hailemariam A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats Advances in Materials Science and Engineering |
| title | A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats |
| title_full | A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats |
| title_fullStr | A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats |
| title_full_unstemmed | A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats |
| title_short | A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats |
| title_sort | combination of coconut fiber suture and tamarind seed gel with dehydrated human amnion membrane for wound surgery in rats |
| url | http://dx.doi.org/10.1155/2021/8122989 |
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