Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection
A rabid, simple and low-cost assay method of histamine-N-methyltransferase activity was developed. Methylhistamine, which was separated from the enzymatic reaction system on reversed-phase high-performance liquid chromatography using an ion-paired chromatographic technique, was detected spectrophoto...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350120093759 |
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| _version_ | 1832545868378537984 |
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| author | Hajime Inoue Ken-Ichi Hisamatsu Miki Yamauchi Norio Kumagai |
| author_facet | Hajime Inoue Ken-Ichi Hisamatsu Miki Yamauchi Norio Kumagai |
| author_sort | Hajime Inoue |
| collection | DOAJ |
| description | A rabid, simple and low-cost assay method of histamine-N-methyltransferase activity was developed. Methylhistamine, which was separated from the enzymatic reaction system on reversed-phase high-performance liquid chromatography using an ion-paired chromatographic technique, was detected spectrophotometrically at 226 nm. The mobile phase used for the separation of methylhistamine was 0.05 M NH4H2 PO4
(pH 3.0) containing 2 mM of sodium octanesulfonate. The new assay technique could detect methylhistamine as an enzyme activity product of histamine-N-methyltransferase in the brain and kidney of rats. Chloropheniramine maleate, an antihistamine, activated the histamine N- methyltransferase. Whether neurotransmitter or neuromodulator, the role of histamine in the brain has not yet been made clear. Therefore, the present method could be applicable for the enzymatic investigation of histamine metabolism in central nervous system or inflammatory reactions. |
| format | Article |
| id | doaj-art-91cbb6fcb632498b8c8ab5040bfe387c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-91cbb6fcb632498b8c8ab5040bfe387c2025-02-03T07:24:32ZengWileyMediators of Inflammation0962-93511466-18612001-01-0110527327710.1080/09629350120093759Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detectionHajime Inoue0Ken-Ichi Hisamatsu1Miki Yamauchi2Norio Kumagai3Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2–16–1 Sugao, Miyamae, Kawasaki 216–8511, JapanDepartment of Otorhinolaryngopharyngology, Surugadai Hospital, Nihon University, 1–18–13 Surugadai, Kanda, Chiyoda, Tokyo 101–8309, JapanDepartment of Otorhinolaryngopharyngology, Surugadai Hospital, Nihon University, 1–18–13 Surugadai, Kanda, Chiyoda, Tokyo 101–8309, JapanDepartment of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, 2–16–1 Sugao, Miyamae, Kawasaki 216–8511, JapanA rabid, simple and low-cost assay method of histamine-N-methyltransferase activity was developed. Methylhistamine, which was separated from the enzymatic reaction system on reversed-phase high-performance liquid chromatography using an ion-paired chromatographic technique, was detected spectrophotometrically at 226 nm. The mobile phase used for the separation of methylhistamine was 0.05 M NH4H2 PO4 (pH 3.0) containing 2 mM of sodium octanesulfonate. The new assay technique could detect methylhistamine as an enzyme activity product of histamine-N-methyltransferase in the brain and kidney of rats. Chloropheniramine maleate, an antihistamine, activated the histamine N- methyltransferase. Whether neurotransmitter or neuromodulator, the role of histamine in the brain has not yet been made clear. Therefore, the present method could be applicable for the enzymatic investigation of histamine metabolism in central nervous system or inflammatory reactions.http://dx.doi.org/10.1080/09629350120093759 |
| spellingShingle | Hajime Inoue Ken-Ichi Hisamatsu Miki Yamauchi Norio Kumagai Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection Mediators of Inflammation |
| title | Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection |
| title_full | Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection |
| title_fullStr | Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection |
| title_full_unstemmed | Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection |
| title_short | Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection |
| title_sort | rapid and simple determination of histamine n methyl transferase activity by high performance liquid chromatography with uv detection |
| url | http://dx.doi.org/10.1080/09629350120093759 |
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