Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang
Walnut (Juglans regia L.) is a good source of lipids and polyunsaturated fatty acids (PUFAs). In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid (FA) synthesis in walnut, the samples at different development periods of three walnut cultivars, ‘Zhipi’ (ZP), ‘X...
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Tsinghua University Press
2022-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000581 |
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| author | Wenqiang Wang Hao Wen Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu |
| author_facet | Wenqiang Wang Hao Wen Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu |
| author_sort | Wenqiang Wang |
| collection | DOAJ |
| description | Walnut (Juglans regia L.) is a good source of lipids and polyunsaturated fatty acids (PUFAs). In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid (FA) synthesis in walnut, the samples at different development periods of three walnut cultivars, ‘Zhipi’ (ZP), ‘Xinwu 417’ (W417) and ‘Xinwen 81’ (W81) were collected for transcriptomic analysis. The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70 %, and over 90 % of FAs were PUFAs. We identified 126 candidate genes including 64 genes for FA de novo synthesis, 45 genes for triacylglycerol assembly, and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing. Ten key enzymes including ACCase, LACS6, LACS8, SAD, FAD2, FAD3, LPAAT1, DGAT2, PDAT2, and PLC encoded by 19 genes were highly associated with lipid biosynthesis. Quantitative PCR analysis further validated 9 important genes, and the results were well consistent with our transcriptomic data. Finally, 5 important transcription factors including WRI1, ABI3, FUS3, PKL and VAL1 were identified, and their main regulatory genes might contain ACCase, KASⅡ, LACS, FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples. These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut. |
| format | Article |
| id | doaj-art-91b947e57eb74c699e9278b8b095eebf |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-91b947e57eb74c699e9278b8b095eebf2025-02-03T05:16:11ZengTsinghua University PressFood Science and Human Wellness2213-45302022-09-0111512011214Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in XinjiangWenqiang Wang0Hao Wen1Qiang Jin2Wenjun Yu3Gen Li4Minyu Wu5Hongjin Bai6Lirong Shen7Cuiyun Wu8Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, ChinaDepartment of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, ChinaCollege of Horticulture and Forestry, Tarim University, Alar 843300, ChinaCollege of Horticulture and Forestry, Tarim University, Alar 843300, ChinaCollege of Horticulture and Forestry, Tarim University, Alar 843300, ChinaDepartment of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, ChinaCollege of Horticulture and Forestry, Tarim University, Alar 843300, ChinaDepartment of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; Corresponding authors at: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; College of Horticulture and Forestry, Tarim University, Alar 843300, China.College of Horticulture and Forestry, Tarim University, Alar 843300, China; Corresponding authors at: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China; College of Horticulture and Forestry, Tarim University, Alar 843300, China.Walnut (Juglans regia L.) is a good source of lipids and polyunsaturated fatty acids (PUFAs). In order to explore the biosynthesis molecular mechanism of oil accumulation and fatty acid (FA) synthesis in walnut, the samples at different development periods of three walnut cultivars, ‘Zhipi’ (ZP), ‘Xinwu 417’ (W417) and ‘Xinwen 81’ (W81) were collected for transcriptomic analysis. The analysis of oil accumulation and FA profiles showed that the oil content in mature walnut kernel was nearly 70 %, and over 90 % of FAs were PUFAs. We identified 126 candidate genes including 64 genes for FA de novo synthesis, 45 genes for triacylglycerol assembly, and 17 genes for oil bodies involved in lipid biosynthesis by RNA-sequencing. Ten key enzymes including ACCase, LACS6, LACS8, SAD, FAD2, FAD3, LPAAT1, DGAT2, PDAT2, and PLC encoded by 19 genes were highly associated with lipid biosynthesis. Quantitative PCR analysis further validated 9 important genes, and the results were well consistent with our transcriptomic data. Finally, 5 important transcription factors including WRI1, ABI3, FUS3, PKL and VAL1 were identified, and their main regulatory genes might contain ACCase, KASⅡ, LACS, FAD3 and LPAAT which were determined through correlation analysis of expression levels for 27 walnut samples. These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in walnut.http://www.sciencedirect.com/science/article/pii/S2213453022000581WalnutOil accumulationTriacylglycerol assemblyTranscription factorsCorrelation analysis |
| spellingShingle | Wenqiang Wang Hao Wen Qiang Jin Wenjun Yu Gen Li Minyu Wu Hongjin Bai Lirong Shen Cuiyun Wu Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang Food Science and Human Wellness Walnut Oil accumulation Triacylglycerol assembly Transcription factors Correlation analysis |
| title | Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang |
| title_full | Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang |
| title_fullStr | Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang |
| title_full_unstemmed | Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang |
| title_short | Comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in Xinjiang |
| title_sort | comparative transcriptome analysis on candidate genes involved in lipid biosynthesis of developing kernels for three walnut cultivars in xinjiang |
| topic | Walnut Oil accumulation Triacylglycerol assembly Transcription factors Correlation analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000581 |
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