Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects
Atopic dermatitis (AD) is a chronic relapsing dermatosis that demands new therapies. This research group previously developed a physically extracted adipose-derived extracellular matrix named adipose collagen fragments (ACF), which was determined containing massive adipose matrix-bound adipokines an...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2025-02-01
|
| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314241312511 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832086520427708416 |
|---|---|
| author | Jingyan Guan Kaiqi Chen Feng Lu Yunfan He |
| author_facet | Jingyan Guan Kaiqi Chen Feng Lu Yunfan He |
| author_sort | Jingyan Guan |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a chronic relapsing dermatosis that demands new therapies. This research group previously developed a physically extracted adipose-derived extracellular matrix named adipose collagen fragments (ACF), which was determined containing massive adipose matrix-bound adipokines and medicable on AD through intradermal injection. However, problems concerning the control of drug release and inevitable pain caused by injection hinder the application of ACF in clinics. Microneedle (MN) is a rapid developing technique for precise and painless transdermal drug delivery. Therefore, a dissolving methacrylated gelatin/hyaluronic acid MN patch loaded with ACF was developed in this study. The morphological characteristics, mechanical properties, penetration ability, as well as biocompatibility and degradation efficiency of ACF-MN were evaluated, and its efficacy on ovalbumin-induced AD mice was also investigated. ACF-MN exhibited excellent penetration ability, biocompatibility, degradation efficiency, and satisfying efficacy on murine AD similar with fresh-made ACF. Furthermore, RNA-Seq combining bioinformatics were performed for mechanism exploration. ACF treatment showed a comprehensive efficacy on AD via restoring inflammatory dysregulation, microbiota imbalance, and skin barrier defects. This study offered a novel MN-based ACF-bound adipokines transdermal delivery system that may serve as a promising strategy for relieving AD. |
| format | Article |
| id | doaj-art-91b664be3f29437f9c9c793af7279e24 |
| institution | Kabale University |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-91b664be3f29437f9c9c793af7279e242025-02-06T13:03:20ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-02-011610.1177/20417314241312511Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defectsJingyan GuanKaiqi ChenFeng LuYunfan HeAtopic dermatitis (AD) is a chronic relapsing dermatosis that demands new therapies. This research group previously developed a physically extracted adipose-derived extracellular matrix named adipose collagen fragments (ACF), which was determined containing massive adipose matrix-bound adipokines and medicable on AD through intradermal injection. However, problems concerning the control of drug release and inevitable pain caused by injection hinder the application of ACF in clinics. Microneedle (MN) is a rapid developing technique for precise and painless transdermal drug delivery. Therefore, a dissolving methacrylated gelatin/hyaluronic acid MN patch loaded with ACF was developed in this study. The morphological characteristics, mechanical properties, penetration ability, as well as biocompatibility and degradation efficiency of ACF-MN were evaluated, and its efficacy on ovalbumin-induced AD mice was also investigated. ACF-MN exhibited excellent penetration ability, biocompatibility, degradation efficiency, and satisfying efficacy on murine AD similar with fresh-made ACF. Furthermore, RNA-Seq combining bioinformatics were performed for mechanism exploration. ACF treatment showed a comprehensive efficacy on AD via restoring inflammatory dysregulation, microbiota imbalance, and skin barrier defects. This study offered a novel MN-based ACF-bound adipokines transdermal delivery system that may serve as a promising strategy for relieving AD.https://doi.org/10.1177/20417314241312511 |
| spellingShingle | Jingyan Guan Kaiqi Chen Feng Lu Yunfan He Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects Journal of Tissue Engineering |
| title | Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects |
| title_full | Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects |
| title_fullStr | Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects |
| title_full_unstemmed | Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects |
| title_short | Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects |
| title_sort | dissolving microneedle patch loaded with adipokines enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders microbiota imbalance and skin barrier defects |
| url | https://doi.org/10.1177/20417314241312511 |
| work_keys_str_mv | AT jingyanguan dissolvingmicroneedlepatchloadedwithadipokinesenrichedadiposeextractrelievesatopicdermatitisinmouseviamodulatingimmunedisordersmicrobiotaimbalanceandskinbarrierdefects AT kaiqichen dissolvingmicroneedlepatchloadedwithadipokinesenrichedadiposeextractrelievesatopicdermatitisinmouseviamodulatingimmunedisordersmicrobiotaimbalanceandskinbarrierdefects AT fenglu dissolvingmicroneedlepatchloadedwithadipokinesenrichedadiposeextractrelievesatopicdermatitisinmouseviamodulatingimmunedisordersmicrobiotaimbalanceandskinbarrierdefects AT yunfanhe dissolvingmicroneedlepatchloadedwithadipokinesenrichedadiposeextractrelievesatopicdermatitisinmouseviamodulatingimmunedisordersmicrobiotaimbalanceandskinbarrierdefects |