The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry
The effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Böhm–Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types:...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/893975 |
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| _version_ | 1832560258646540288 |
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| author | Margareta Fležar Alexei Doudkine Marija Us-Krašovec |
| author_facet | Margareta Fležar Alexei Doudkine Marija Us-Krašovec |
| author_sort | Margareta Fležar |
| collection | DOAJ |
| description | The effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Böhm–Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types: lymphatic cells (tonsil), epithelial cells (buccal mucosa) and mesenchymal cells (uterine myometrium) were used for the study. Our findings show, that generally not all features have the same plateau times as the IOD (integrated optical density), and that many features show different values depending on cell type and fixation method. It is therefore recommended that for any primary fixative used in routine clinical work and for each cell type, the hydrolysis curve for all nuclear features to be used in sample analysis should be established. |
| format | Article |
| id | doaj-art-91a73075cd3546f8a62991ac87b2f83f |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-91a73075cd3546f8a62991ac87b2f83f2025-02-03T01:28:01ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0117313114410.1155/1998/893975The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image CytometryMargareta Fležar0Alexei Doudkine1Marija Us-Krašovec2Department Cytopathology, Institute of Oncology, 1000 Ljubljana, SloveniaOncometrics Imaging Corp., Vancouver, BC, V5Z 1L3, CanadaDepartment Cytopathology, Institute of Oncology, 1000 Ljubljana, SloveniaThe effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Böhm–Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types: lymphatic cells (tonsil), epithelial cells (buccal mucosa) and mesenchymal cells (uterine myometrium) were used for the study. Our findings show, that generally not all features have the same plateau times as the IOD (integrated optical density), and that many features show different values depending on cell type and fixation method. It is therefore recommended that for any primary fixative used in routine clinical work and for each cell type, the hydrolysis curve for all nuclear features to be used in sample analysis should be established.http://dx.doi.org/10.1155/1998/893975 |
| spellingShingle | Margareta Fležar Alexei Doudkine Marija Us-Krašovec The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry Analytical Cellular Pathology |
| title | The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry |
| title_full | The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry |
| title_fullStr | The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry |
| title_full_unstemmed | The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry |
| title_short | The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image Cytometry |
| title_sort | effect of primary fixation with standard postfixation and the duration of hydrolysis on nuclear features in image cytometry |
| url | http://dx.doi.org/10.1155/1998/893975 |
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