Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects
Spermatogenesis is a fascinating biological process aiming to generate haploid spermatozoa from diploid spermatogonia through a specific hormonal network between gonadotropins and steroids. Increasing evidence suggests that the primary female sex hormone estrogen plays an active role in this process...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2017/4760638 |
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| author | Mariailaria Verderame Rosaria Scudiero Ermelinda Limatola |
| author_facet | Mariailaria Verderame Rosaria Scudiero Ermelinda Limatola |
| author_sort | Mariailaria Verderame |
| collection | DOAJ |
| description | Spermatogenesis is a fascinating biological process aiming to generate haploid spermatozoa from diploid spermatogonia through a specific hormonal network between gonadotropins and steroids. Increasing evidence suggests that the primary female sex hormone estrogen plays an active role in this process. This research points out on the role of estrogen during lizard spermatogenesis by using three experimental approaches: (1) exposure to an analogue of nonsteroidal estrogen as Clomiphene citrate that acts both as estrogen agonist and antagonist; (2) exposure to the gonadotropin FSH; and (3) exposures to FSH followed by Clomiphene. Histological and immunohistochemical results demonstrate that in the lizard Podarcis sicula during the mating period, Clomiphene as well as FSH determines the breakdown of spermatogenesis and the epididymal regression, presumably through estrogens input as indirectly demonstrated by the appearance of ERα and vitellogenin in the liver. The ability of Clomiphene to restore the gonadal natural condition after FSH treatment is also demonstrated. Finally, data indicate that lizard testis and epididymis control their morphophysiology regulating the intracellular presence of ERα. |
| format | Article |
| id | doaj-art-9190382a9e784fcd8127affce25d9c3f |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-9190382a9e784fcd8127affce25d9c3f2025-02-03T01:33:06ZengWileyInternational Journal of Endocrinology1687-83371687-83452017-01-01201710.1155/2017/47606384760638Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH EffectsMariailaria Verderame0Rosaria Scudiero1Ermelinda Limatola2Department of Biology, University Federico II, Via Mezzocannone 8, 80134 Naples, ItalyDepartment of Biology, University Federico II, Via Mezzocannone 8, 80134 Naples, ItalyDepartment of Biology, University Federico II, Via Mezzocannone 8, 80134 Naples, ItalySpermatogenesis is a fascinating biological process aiming to generate haploid spermatozoa from diploid spermatogonia through a specific hormonal network between gonadotropins and steroids. Increasing evidence suggests that the primary female sex hormone estrogen plays an active role in this process. This research points out on the role of estrogen during lizard spermatogenesis by using three experimental approaches: (1) exposure to an analogue of nonsteroidal estrogen as Clomiphene citrate that acts both as estrogen agonist and antagonist; (2) exposure to the gonadotropin FSH; and (3) exposures to FSH followed by Clomiphene. Histological and immunohistochemical results demonstrate that in the lizard Podarcis sicula during the mating period, Clomiphene as well as FSH determines the breakdown of spermatogenesis and the epididymal regression, presumably through estrogens input as indirectly demonstrated by the appearance of ERα and vitellogenin in the liver. The ability of Clomiphene to restore the gonadal natural condition after FSH treatment is also demonstrated. Finally, data indicate that lizard testis and epididymis control their morphophysiology regulating the intracellular presence of ERα.http://dx.doi.org/10.1155/2017/4760638 |
| spellingShingle | Mariailaria Verderame Rosaria Scudiero Ermelinda Limatola Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects International Journal of Endocrinology |
| title | Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects |
| title_full | Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects |
| title_fullStr | Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects |
| title_full_unstemmed | Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects |
| title_short | Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects |
| title_sort | exploring the role of estrogens in lizard spermatogenesis through the study of clomiphene and fsh effects |
| url | http://dx.doi.org/10.1155/2017/4760638 |
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