Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i>
Terpenes are critical components of the floral fragrance component in <i>Dendrobium chrysotoxum</i>, synthesized by terpene synthase (TPS). Analysis of the <i>D. chrysotoxum</i> genome and transcriptional data revealed that the gene <i>DcTPSb1</i> was significantl...
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2025-01-01
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| author | Yuxuan Jin Shuting Zhou Zhihui Du Weize Wang Zhilin Chen |
| author_facet | Yuxuan Jin Shuting Zhou Zhihui Du Weize Wang Zhilin Chen |
| author_sort | Yuxuan Jin |
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| description | Terpenes are critical components of the floral fragrance component in <i>Dendrobium chrysotoxum</i>, synthesized by terpene synthase (TPS). Analysis of the <i>D. chrysotoxum</i> genome and transcriptional data revealed that the gene <i>DcTPSb1</i> was significantly up-regulated during flowering periods, showing a strong correlation with the accumulation of aromatic monoterpenes in the floral components of <i>Dendrobium chrysotoxum</i>. Consequently, the <i>DcTPSb1</i> gene was selected for further analysis. <i>DcTPSb1</i> exhibited elevated expression levels in flowers among four organs (roots, stems, leaves, flowers) of <i>D. chrysotoxum</i>, with the highest expression observed during the blooming phase, which aligned with the accumulation of volatile terpenes during flowering. DcTPSb1, located in the chloroplasts, was identified as a member of the TPS-b subfamily associated with monoterpenes synthesis, showing close phylogenetic relationships with homologous proteins in related plant species. An analysis of the promoter region of <i>DcTPSb1</i> indicated that it may be regulated by methyl jasmonate (MeJA) responsiveness. Functionally, DcTPSb1 was shown to catalyze the conversion of geranyl diphosphate (GPP) to linalool, ocimene, and (-)-α-pinitol in vitro. Overexpression of <i>DcTPSb1</i> in tobacco resulted in a significant increase in terpenoid release during the blooming stage; however, the up-regulated substances did not include their catalytic products. The classification of DcTPSb1 as a terpene synthase capable of producing multiple products provides valuable insights into the complex biosynthesis of terpenes in orchids. These findings enhance our understanding of the functional diversity of <i>DcTPSb1</i> and the processes involved in terpene biosynthesis in orchids. |
| format | Article |
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| spelling | doaj-art-9169fcb34fc7478d92278cfa61fd31a52025-01-24T13:27:26ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-01-014712510.3390/cimb47010025Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i>Yuxuan Jin0Shuting Zhou1Zhihui Du2Weize Wang3Zhilin Chen4Guizhou Horticulture Institute/Horticultural Engineering Technology Research Center of Guizhou, Guizhou Academy of Agricultural Sciences, Guiyang 550000, ChinaNatural Products Research Center of Guizhou Province, Guiyang 550000, ChinaGuizhou Horticulture Institute/Horticultural Engineering Technology Research Center of Guizhou, Guizhou Academy of Agricultural Sciences, Guiyang 550000, ChinaGuizhou Horticulture Institute/Horticultural Engineering Technology Research Center of Guizhou, Guizhou Academy of Agricultural Sciences, Guiyang 550000, ChinaGuizhou Horticulture Institute/Horticultural Engineering Technology Research Center of Guizhou, Guizhou Academy of Agricultural Sciences, Guiyang 550000, ChinaTerpenes are critical components of the floral fragrance component in <i>Dendrobium chrysotoxum</i>, synthesized by terpene synthase (TPS). Analysis of the <i>D. chrysotoxum</i> genome and transcriptional data revealed that the gene <i>DcTPSb1</i> was significantly up-regulated during flowering periods, showing a strong correlation with the accumulation of aromatic monoterpenes in the floral components of <i>Dendrobium chrysotoxum</i>. Consequently, the <i>DcTPSb1</i> gene was selected for further analysis. <i>DcTPSb1</i> exhibited elevated expression levels in flowers among four organs (roots, stems, leaves, flowers) of <i>D. chrysotoxum</i>, with the highest expression observed during the blooming phase, which aligned with the accumulation of volatile terpenes during flowering. DcTPSb1, located in the chloroplasts, was identified as a member of the TPS-b subfamily associated with monoterpenes synthesis, showing close phylogenetic relationships with homologous proteins in related plant species. An analysis of the promoter region of <i>DcTPSb1</i> indicated that it may be regulated by methyl jasmonate (MeJA) responsiveness. Functionally, DcTPSb1 was shown to catalyze the conversion of geranyl diphosphate (GPP) to linalool, ocimene, and (-)-α-pinitol in vitro. Overexpression of <i>DcTPSb1</i> in tobacco resulted in a significant increase in terpenoid release during the blooming stage; however, the up-regulated substances did not include their catalytic products. The classification of DcTPSb1 as a terpene synthase capable of producing multiple products provides valuable insights into the complex biosynthesis of terpenes in orchids. These findings enhance our understanding of the functional diversity of <i>DcTPSb1</i> and the processes involved in terpene biosynthesis in orchids.https://www.mdpi.com/1467-3045/47/1/25<i>Dendrobium chrysotoxum</i><i>DcTPSb1</i>terpene synthasefloral fragrance |
| spellingShingle | Yuxuan Jin Shuting Zhou Zhihui Du Weize Wang Zhilin Chen Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> Current Issues in Molecular Biology <i>Dendrobium chrysotoxum</i> <i>DcTPSb1</i> terpene synthase floral fragrance |
| title | Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> |
| title_full | Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> |
| title_fullStr | Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> |
| title_full_unstemmed | Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> |
| title_short | Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> |
| title_sort | expression analysis and functional validation of dctpsb1 in terpene synthesis of i dendrobium chrysotoxum i |
| topic | <i>Dendrobium chrysotoxum</i> <i>DcTPSb1</i> terpene synthase floral fragrance |
| url | https://www.mdpi.com/1467-3045/47/1/25 |
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