Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System
A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. The objective of this study was to invest...
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MDPI AG
2025-01-01
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| author | Hai-Jun Liu Serena Jocelyn Wai Yin Oh Nicole Liling Tay Christina Yingyan Lim Chia-Da Hsu Delia Hwee Hoon Chua Winnie Koon Lay Teo Yuin-Han Loh Soon Chye Ng |
| author_facet | Hai-Jun Liu Serena Jocelyn Wai Yin Oh Nicole Liling Tay Christina Yingyan Lim Chia-Da Hsu Delia Hwee Hoon Chua Winnie Koon Lay Teo Yuin-Han Loh Soon Chye Ng |
| author_sort | Hai-Jun Liu |
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| description | A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. The objective of this study was to investigate the morphokinetic parameters of fishing cat–domestic cat interspecies somatic cell nuclear transfer (iSCNT) embryos from one-cell to blastocyst stages, and in particular, the cleavage patterns of the first division in iSCNT and IVF embryos, as these play a central role in euploidy. Domestic cat in vitro fertilization (IVF) embryos were set up as controls. The results show that morula and blastocyst development rates were significantly lower in the iSCNT embryos compared to their IVF counterparts. All earlier time points of embryonic development before the onset of blastulation in the iSCNT embryos were significantly delayed when compared with their IVF counterparts. In iSCNT, normal embryos (defined as those that developed to the blastocyst stage) took a longer time to reach the morula stage, and these morulas were more likely to undergo compaction, compared to their arrested embryo counterparts. Direct cleavage in the first division is a morphological aberration, and was seen with greater prevalence in iSCNT embryos than control IVF embryos; these aberrant embryos displayed a significantly lower blastocyst development rate than embryos that had undergone normal cleavage. In conclusion, the morphokinetic parameters of fishing cat–domestic cat iSCNT embryos at early stages could be used to predict their potential for development to the blastocyst stage. A time-lapse imaging system is potentially a powerful tool for selecting early embryos with developmental potential for transfer, and hence, for improving feline iSCNT efficiency. |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-9158f26ec6d644abaa00af66501548f72025-01-24T13:17:45ZengMDPI AGAnimals2076-26152025-01-0115214810.3390/ani15020148Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse SystemHai-Jun Liu0Serena Jocelyn Wai Yin Oh1Nicole Liling Tay2Christina Yingyan Lim3Chia-Da Hsu4Delia Hwee Hoon Chua5Winnie Koon Lay Teo6Yuin-Han Loh7Soon Chye Ng8Endangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeMandai Wildlife Group, 80 Mandai Lake Road, Singapore 729826, SingaporeMandai Wildlife Group, 80 Mandai Lake Road, Singapore 729826, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeEndangered Species Conservation via Assisted Reproduction (ESCAR) Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673, SingaporeA time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. The objective of this study was to investigate the morphokinetic parameters of fishing cat–domestic cat interspecies somatic cell nuclear transfer (iSCNT) embryos from one-cell to blastocyst stages, and in particular, the cleavage patterns of the first division in iSCNT and IVF embryos, as these play a central role in euploidy. Domestic cat in vitro fertilization (IVF) embryos were set up as controls. The results show that morula and blastocyst development rates were significantly lower in the iSCNT embryos compared to their IVF counterparts. All earlier time points of embryonic development before the onset of blastulation in the iSCNT embryos were significantly delayed when compared with their IVF counterparts. In iSCNT, normal embryos (defined as those that developed to the blastocyst stage) took a longer time to reach the morula stage, and these morulas were more likely to undergo compaction, compared to their arrested embryo counterparts. Direct cleavage in the first division is a morphological aberration, and was seen with greater prevalence in iSCNT embryos than control IVF embryos; these aberrant embryos displayed a significantly lower blastocyst development rate than embryos that had undergone normal cleavage. In conclusion, the morphokinetic parameters of fishing cat–domestic cat iSCNT embryos at early stages could be used to predict their potential for development to the blastocyst stage. A time-lapse imaging system is potentially a powerful tool for selecting early embryos with developmental potential for transfer, and hence, for improving feline iSCNT efficiency.https://www.mdpi.com/2076-2615/15/2/148interspecies somatic cell nuclear transfer embryosmorphokinetic parameterscleavage patternsfishing cat |
| spellingShingle | Hai-Jun Liu Serena Jocelyn Wai Yin Oh Nicole Liling Tay Christina Yingyan Lim Chia-Da Hsu Delia Hwee Hoon Chua Winnie Koon Lay Teo Yuin-Han Loh Soon Chye Ng Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System Animals interspecies somatic cell nuclear transfer embryos morphokinetic parameters cleavage patterns fishing cat |
| title | Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System |
| title_full | Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System |
| title_fullStr | Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System |
| title_full_unstemmed | Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System |
| title_short | Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System |
| title_sort | morphokinetic analyses of fishing cat domestic cat interspecies somatic cell nuclear transfer embryos through a time lapse system |
| topic | interspecies somatic cell nuclear transfer embryos morphokinetic parameters cleavage patterns fishing cat |
| url | https://www.mdpi.com/2076-2615/15/2/148 |
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