In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery
Oil-in-water nanoemulsions (NEs) represent one of the formulation approaches to improve eye-related bio-availability of lipophilic drugs. The potential of cationic NEs is pronounced due to the electrostatic interaction of positively charged droplets with negatively charged mucins present in the tear...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2019-0054 |
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| author | Dukovski Bisera Jurišić Bračko Ana Šare Marija Pepić Ivan Lovrić Jasmina |
| author_facet | Dukovski Bisera Jurišić Bračko Ana Šare Marija Pepić Ivan Lovrić Jasmina |
| author_sort | Dukovski Bisera Jurišić |
| collection | DOAJ |
| description | Oil-in-water nanoemulsions (NEs) represent one of the formulation approaches to improve eye-related bio-availability of lipophilic drugs. The potential of cationic NEs is pronounced due to the electrostatic interaction of positively charged droplets with negatively charged mucins present in the tear film, providing prolonged formulation residence at the ocular surface. The aim of this study was to develop a cationic ophthalmic NE with cationic lipid stearylamine (SA) as a carrier of a positive charge. The addition of a nonionic surfactant provided the dual electro-steric stabilization of NEs and enabled tuning of SA concentration to achieve an optimal balance between its interaction with mucins and biocompatibility. Physicochemical characterization, stability profile, in vitro mucoadhesion study and biocompatibility study employing 3D HCE-T cell-based model of corneal epithelium pointed out the NE with 0.05 % (m/m) SA as the leading formulation. Minimizing SA content while retaining droplet/mucin interactions is of great importance for efficacy and safety of future ophthalmic drug products. |
| format | Article |
| id | doaj-art-912bc061b015423d90381c9dd5221a76 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-912bc061b015423d90381c9dd5221a762025-02-02T11:25:09ZengSciendoActa Pharmaceutica1846-95582019-12-0169462163410.2478/acph-2019-0054acph-2019-0054In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug deliveryDukovski Bisera Jurišić0Bračko Ana1Šare Marija2Pepić Ivan3Lovrić Jasmina4University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, HR-10000Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, HR-10000Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, HR-10000Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, HR-10000Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, HR-10000Zagreb, CroatiaOil-in-water nanoemulsions (NEs) represent one of the formulation approaches to improve eye-related bio-availability of lipophilic drugs. The potential of cationic NEs is pronounced due to the electrostatic interaction of positively charged droplets with negatively charged mucins present in the tear film, providing prolonged formulation residence at the ocular surface. The aim of this study was to develop a cationic ophthalmic NE with cationic lipid stearylamine (SA) as a carrier of a positive charge. The addition of a nonionic surfactant provided the dual electro-steric stabilization of NEs and enabled tuning of SA concentration to achieve an optimal balance between its interaction with mucins and biocompatibility. Physicochemical characterization, stability profile, in vitro mucoadhesion study and biocompatibility study employing 3D HCE-T cell-based model of corneal epithelium pointed out the NE with 0.05 % (m/m) SA as the leading formulation. Minimizing SA content while retaining droplet/mucin interactions is of great importance for efficacy and safety of future ophthalmic drug products.https://doi.org/10.2478/acph-2019-0054cationic nanoemulsionsstearylamineophthalmic drug deliverybiocompatibilitymucoadhesion in vitro |
| spellingShingle | Dukovski Bisera Jurišić Bračko Ana Šare Marija Pepić Ivan Lovrić Jasmina In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery Acta Pharmaceutica cationic nanoemulsions stearylamine ophthalmic drug delivery biocompatibility mucoadhesion in vitro |
| title | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| title_full | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| title_fullStr | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| title_full_unstemmed | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| title_short | In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| title_sort | in vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery |
| topic | cationic nanoemulsions stearylamine ophthalmic drug delivery biocompatibility mucoadhesion in vitro |
| url | https://doi.org/10.2478/acph-2019-0054 |
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