Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B
Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson’s disease (PD) model via a 6-OHDA intrastriatal injectio...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/387028 |
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| author | Cristiane Batassini Núbia Broetto Lucas Silva Tortorelli Milene Borsoi Caroline Zanotto Fabiana Galland Tadeu Mello Souza Marina Concli Leite Carlos-Alberto Gonçalves |
| author_facet | Cristiane Batassini Núbia Broetto Lucas Silva Tortorelli Milene Borsoi Caroline Zanotto Fabiana Galland Tadeu Mello Souza Marina Concli Leite Carlos-Alberto Gonçalves |
| author_sort | Cristiane Batassini |
| collection | DOAJ |
| description | Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson’s disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model. |
| format | Article |
| id | doaj-art-912aaef15d7b4f3bafc193344905ec9b |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-912aaef15d7b4f3bafc193344905ec9b2025-02-03T01:00:37ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/387028387028Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100BCristiane Batassini0Núbia Broetto1Lucas Silva Tortorelli2Milene Borsoi3Caroline Zanotto4Fabiana Galland5Tadeu Mello Souza6Marina Concli Leite7Carlos-Alberto Gonçalves8Biochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilNeuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, BrazilBiochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilNeuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, BrazilBiochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilBiochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilNeuroscience Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90050-170 Porto Alegre, RS, BrazilBiochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilBiochemistry Postgraduate Program, Federal University of Rio Grande do Sul (UFRGS), 90035-003 Porto Alegre, RS, BrazilBoth glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson’s disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model.http://dx.doi.org/10.1155/2015/387028 |
| spellingShingle | Cristiane Batassini Núbia Broetto Lucas Silva Tortorelli Milene Borsoi Caroline Zanotto Fabiana Galland Tadeu Mello Souza Marina Concli Leite Carlos-Alberto Gonçalves Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B Neural Plasticity |
| title | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
| title_full | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
| title_fullStr | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
| title_full_unstemmed | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
| title_short | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
| title_sort | striatal injury with 6 ohda transiently increases cerebrospinal gfap and s100b |
| url | http://dx.doi.org/10.1155/2015/387028 |
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