Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2
The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its trans...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2020/3731953 |
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| _version_ | 1832547162346487808 |
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| author | Leilei Xu Qin Zhang Changhua Li Fu Hua Xiaoping Liu |
| author_facet | Leilei Xu Qin Zhang Changhua Li Fu Hua Xiaoping Liu |
| author_sort | Leilei Xu |
| collection | DOAJ |
| description | The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2. |
| format | Article |
| id | doaj-art-910e472194bf4276a86943b1633b2245 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-910e472194bf4276a86943b1633b22452025-02-03T06:45:50ZengWileyAnalytical Cellular Pathology2210-71772210-71852020-01-01202010.1155/2020/37319533731953Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2Leilei Xu0Qin Zhang1Changhua Li2Fu Hua3Xiaoping Liu4Department of Gynecology, The First Hospital of Hua’an Affiliated to Nanjing Medical University, Huai’an, 223300 Jiangsu, ChinaDepartment of Ultrasound, The First Hospital of Huai’an Affiliated to Nanjing Medical University, Huai’an, 223300 Jiangsu, ChinaDepartment of Gynecology, The First Hospital of Hua’an Affiliated to Nanjing Medical University, Huai’an, 223300 Jiangsu, ChinaDepartment of Gynecology, The First Hospital of Hua’an Affiliated to Nanjing Medical University, Huai’an, 223300 Jiangsu, ChinaDepartment of Gynecology, The First Hospital of Hua’an Affiliated to Nanjing Medical University, Huai’an, 223300 Jiangsu, ChinaThe application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2.http://dx.doi.org/10.1155/2020/3731953 |
| spellingShingle | Leilei Xu Qin Zhang Changhua Li Fu Hua Xiaoping Liu Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 Analytical Cellular Pathology |
| title | Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 |
| title_full | Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 |
| title_fullStr | Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 |
| title_full_unstemmed | Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 |
| title_short | Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2 |
| title_sort | ultrasound microbubble mediated microrna 505 regulates cervical cancer cell growth via akt2 |
| url | http://dx.doi.org/10.1155/2020/3731953 |
| work_keys_str_mv | AT leileixu ultrasoundmicrobubblemediatedmicrorna505regulatescervicalcancercellgrowthviaakt2 AT qinzhang ultrasoundmicrobubblemediatedmicrorna505regulatescervicalcancercellgrowthviaakt2 AT changhuali ultrasoundmicrobubblemediatedmicrorna505regulatescervicalcancercellgrowthviaakt2 AT fuhua ultrasoundmicrobubblemediatedmicrorna505regulatescervicalcancercellgrowthviaakt2 AT xiaopingliu ultrasoundmicrobubblemediatedmicrorna505regulatescervicalcancercellgrowthviaakt2 |