Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines
<i>Clinopodium vulgare</i> L. is a valuable medicinal plant with various beneficial effects on health. In this study, water extracts from the aerial part of the wild and in vitro cultured <i>C. vulgare</i> plants were obtained. The polyphenol, flavonoid content and antioxidan...
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2025-01-01
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| author | Ani Georgieva Inna Sulikovska Tanya Toshkova-Yotova Vera Djeliova Sabrina Amiri Nenad Tsonevski Polina Petkova-Kirova Krasimira Tasheva |
| author_facet | Ani Georgieva Inna Sulikovska Tanya Toshkova-Yotova Vera Djeliova Sabrina Amiri Nenad Tsonevski Polina Petkova-Kirova Krasimira Tasheva |
| author_sort | Ani Georgieva |
| collection | DOAJ |
| description | <i>Clinopodium vulgare</i> L. is a valuable medicinal plant with various beneficial effects on health. In this study, water extracts from the aerial part of the wild and in vitro cultured <i>C. vulgare</i> plants were obtained. The polyphenol, flavonoid content and antioxidant activity of the extracts as well as their antitumor efficiency against a panel of cell lines were analyzed. The ability of <i>C. vulgare</i> to inhibit cancer cell migration and induce apoptosis in the tumor cells was examined by wound healing assay and fluorescence microscopic methods. The effect of the extracts on the cell cycle progression of the tumor cells was analyzed by flow cytometry. The presented results show that the antitumor activity of the extracts from in vitro cultured plants was similar to and even exceeded that of the wild plants. The cell viability and migration assays demonstrate the selective anticancer effect of the extract and significant inhibition of cancer cell proliferation and motility. The fluorescence microscopy and cell cycle analyses indicate that the antitumor activity of the in vitro plant extract was related to both antiproliferative and proapoptotic effects. These results show that <i>C. vulgare</i> plants obtained by in vitro micropropagation and cultivated ex vitro are promising candidates for anticancer drug therapy. |
| format | Article |
| id | doaj-art-9103ae474c484651a091063ab47f5350 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-9103ae474c484651a091063ab47f53502025-01-24T13:21:21ZengMDPI AGApplied Sciences2076-34172025-01-0115292510.3390/app15020925Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell LinesAni Georgieva0Inna Sulikovska1Tanya Toshkova-Yotova2Vera Djeliova3Sabrina Amiri4Nenad Tsonevski5Polina Petkova-Kirova6Krasimira Tasheva7Department of Pathology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 25, 1113 Sofia, BulgariaDepartment of Pathology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 25, 1113 Sofia, BulgariaDepartment of Plant Ecophysiology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 21, 1113 Sofia, BulgariaDepartment of Molecular Biology of Cell Cycle, Institute of Molecular Biology “Acad. R. Tsanev”, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 21, 1113 Sofia, BulgariaClinic of Thoracic Surgery, Military Medical Academy, Georgi Sofiyski Str., 3, 1606 Sofia, BulgariaClinic of Thoracic Surgery, Military Medical Academy, Georgi Sofiyski Str., 3, 1606 Sofia, BulgariaDepartment of Synaptic Signaling and Communication, Institute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 23, 1113 Sofia, BulgariaDepartment of Plant Ecophysiology, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., 21, 1113 Sofia, Bulgaria<i>Clinopodium vulgare</i> L. is a valuable medicinal plant with various beneficial effects on health. In this study, water extracts from the aerial part of the wild and in vitro cultured <i>C. vulgare</i> plants were obtained. The polyphenol, flavonoid content and antioxidant activity of the extracts as well as their antitumor efficiency against a panel of cell lines were analyzed. The ability of <i>C. vulgare</i> to inhibit cancer cell migration and induce apoptosis in the tumor cells was examined by wound healing assay and fluorescence microscopic methods. The effect of the extracts on the cell cycle progression of the tumor cells was analyzed by flow cytometry. The presented results show that the antitumor activity of the extracts from in vitro cultured plants was similar to and even exceeded that of the wild plants. The cell viability and migration assays demonstrate the selective anticancer effect of the extract and significant inhibition of cancer cell proliferation and motility. The fluorescence microscopy and cell cycle analyses indicate that the antitumor activity of the in vitro plant extract was related to both antiproliferative and proapoptotic effects. These results show that <i>C. vulgare</i> plants obtained by in vitro micropropagation and cultivated ex vitro are promising candidates for anticancer drug therapy.https://www.mdpi.com/2076-3417/15/2/925<i>Clinopodium vulgare</i>in vitro cultured plantsantitumor activitycell viabilityapoptosis |
| spellingShingle | Ani Georgieva Inna Sulikovska Tanya Toshkova-Yotova Vera Djeliova Sabrina Amiri Nenad Tsonevski Polina Petkova-Kirova Krasimira Tasheva Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines Applied Sciences <i>Clinopodium vulgare</i> in vitro cultured plants antitumor activity cell viability apoptosis |
| title | Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines |
| title_full | Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines |
| title_fullStr | Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines |
| title_full_unstemmed | Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines |
| title_short | Antitumor Activity of Whole-Plant Extracts from In Vitro Cultured and Wild-Growing <i>Clinopodium vulgare</i> Plants on a Panel of Human Tumor Cell Lines |
| title_sort | antitumor activity of whole plant extracts from in vitro cultured and wild growing i clinopodium vulgare i plants on a panel of human tumor cell lines |
| topic | <i>Clinopodium vulgare</i> in vitro cultured plants antitumor activity cell viability apoptosis |
| url | https://www.mdpi.com/2076-3417/15/2/925 |
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