A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets
Abstract This study aims to investigate the expression of seven cancer testis antigens (MAGE-A1, MAGE-A4, MAGE-A10, MAGE-A11, PRAME, NY-ESO-1 and KK-LC-1) in pan squamous cell carcinoma and their prognostic value, thus assessing the potential of these CTAs as immunotherapeutic targets. The protein e...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Oncology |
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| Online Access: | https://doi.org/10.1007/s12672-025-01804-z |
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| author | Lin Li Xin Zhang Jiayao Yan Jingyi Guo Fangcen Liu Xiao Wei Qin Liu Kongcheng Wang Baorui Liu |
| author_facet | Lin Li Xin Zhang Jiayao Yan Jingyi Guo Fangcen Liu Xiao Wei Qin Liu Kongcheng Wang Baorui Liu |
| author_sort | Lin Li |
| collection | DOAJ |
| description | Abstract This study aims to investigate the expression of seven cancer testis antigens (MAGE-A1, MAGE-A4, MAGE-A10, MAGE-A11, PRAME, NY-ESO-1 and KK-LC-1) in pan squamous cell carcinoma and their prognostic value, thus assessing the potential of these CTAs as immunotherapeutic targets. The protein expression of these CTAs was evaluated by immunohistochemistry in 60 lung squamous cell carcinoma (LUSC), 62 esophageal squamous cell carcinoma (ESCA) and 62 head and neck squamous cell carcinoma (HNSC). The relationship between CTAs expression and progression-free survival (PFS) was assessed. PD-L1 expression and tumor-infiltrating lymphocytes were also collected and correlated with CTAs expression. The prognostic impact of CTAs gene expression was evaluated using the Kaplan–Meier Plotter website. CTAs expression was 0–48% in ESCA, 3%-77% in LUSC, and 3%-71% in HNSC. Analysis of PFS showed that MAGE-A1 expression in HNSC (**p < 0.01), PRAME in LUSC (p = 0.008, **p < 0.01), MAGE-A10 (p = 0.012, *p < 0.05) and PRAME (p = 0.021, *p < 0.05) in ESCA were significantly correlated with PFS. In all three cancers, coexpression of three CTAs was used as a cutoff value for grouping, and the results showed a significant difference in PFS between these two groups. Moreover, CTAs expression was significantly correlated with PD-L1 expression and T cell infiltration. These findings indicate a high incidence of CTA expression in HNSC, LUSC and ESCA, which was correlated with PD-L1 expression, T cell infiltration, and tumor progression. The results suggest that cancer testis antigens could be feasible vaccine targets in squamous cell carcinoma. |
| format | Article |
| id | doaj-art-90fbae194ec34270b32f5caff02972dc |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-90fbae194ec34270b32f5caff02972dc2025-01-26T12:39:45ZengSpringerDiscover Oncology2730-60112025-01-0116111410.1007/s12672-025-01804-zA panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targetsLin Li0Xin Zhang1Jiayao Yan2Jingyi Guo3Fangcen Liu4Xiao Wei5Qin Liu6Kongcheng Wang7Baorui Liu8Department of Pathology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Pathology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of China Pharmaceutical UniversityDepartment of Pathology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Pathology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Oncology, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineAbstract This study aims to investigate the expression of seven cancer testis antigens (MAGE-A1, MAGE-A4, MAGE-A10, MAGE-A11, PRAME, NY-ESO-1 and KK-LC-1) in pan squamous cell carcinoma and their prognostic value, thus assessing the potential of these CTAs as immunotherapeutic targets. The protein expression of these CTAs was evaluated by immunohistochemistry in 60 lung squamous cell carcinoma (LUSC), 62 esophageal squamous cell carcinoma (ESCA) and 62 head and neck squamous cell carcinoma (HNSC). The relationship between CTAs expression and progression-free survival (PFS) was assessed. PD-L1 expression and tumor-infiltrating lymphocytes were also collected and correlated with CTAs expression. The prognostic impact of CTAs gene expression was evaluated using the Kaplan–Meier Plotter website. CTAs expression was 0–48% in ESCA, 3%-77% in LUSC, and 3%-71% in HNSC. Analysis of PFS showed that MAGE-A1 expression in HNSC (**p < 0.01), PRAME in LUSC (p = 0.008, **p < 0.01), MAGE-A10 (p = 0.012, *p < 0.05) and PRAME (p = 0.021, *p < 0.05) in ESCA were significantly correlated with PFS. In all three cancers, coexpression of three CTAs was used as a cutoff value for grouping, and the results showed a significant difference in PFS between these two groups. Moreover, CTAs expression was significantly correlated with PD-L1 expression and T cell infiltration. These findings indicate a high incidence of CTA expression in HNSC, LUSC and ESCA, which was correlated with PD-L1 expression, T cell infiltration, and tumor progression. The results suggest that cancer testis antigens could be feasible vaccine targets in squamous cell carcinoma.https://doi.org/10.1007/s12672-025-01804-zCancer testis antigensHead and neck squamous cell carcinomaLung squamous cell carcinomaEsophageal squamous cell carcinomaImmunotherapyPD-L1 |
| spellingShingle | Lin Li Xin Zhang Jiayao Yan Jingyi Guo Fangcen Liu Xiao Wei Qin Liu Kongcheng Wang Baorui Liu A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets Discover Oncology Cancer testis antigens Head and neck squamous cell carcinoma Lung squamous cell carcinoma Esophageal squamous cell carcinoma Immunotherapy PD-L1 |
| title | A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets |
| title_full | A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets |
| title_fullStr | A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets |
| title_full_unstemmed | A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets |
| title_short | A panel of cancer testis antigens in squamous cell carcinoma of the lung, head and neck, and esophagus: implication for biomarkers and therapeutic targets |
| title_sort | panel of cancer testis antigens in squamous cell carcinoma of the lung head and neck and esophagus implication for biomarkers and therapeutic targets |
| topic | Cancer testis antigens Head and neck squamous cell carcinoma Lung squamous cell carcinoma Esophageal squamous cell carcinoma Immunotherapy PD-L1 |
| url | https://doi.org/10.1007/s12672-025-01804-z |
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