Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations
Three simple, sensitive and cost effective Spectrophotometric methods are described for the determination of pitavastatin calcium (PST) in bulk drugs and in pharmaceutical formulations. These methods are based on the oxidation of PST by ferric chloride in presence of o-phenanthroline (Method A) or 2...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2007/628987 |
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| author | Marothu Vamsi Krishna Dannana Gowri Sankar |
| author_facet | Marothu Vamsi Krishna Dannana Gowri Sankar |
| author_sort | Marothu Vamsi Krishna |
| collection | DOAJ |
| description | Three simple, sensitive and cost effective Spectrophotometric methods are described for the determination of pitavastatin calcium (PST) in bulk drugs and in pharmaceutical formulations. These methods are based on the oxidation of PST by ferric chloride in presence of o-phenanthroline (Method A) or 2, 2’ bipyridyl (Method B) or potassium ferricyanide (Method C). The colored complex formed was measured at 510, 530 and 755 nm for method A, B and C respectively against the reagent blank prepared in the same manner. The optimum experimental parameters for the color production are selected. Beer’s law is valid with in a concentration range of 4-20 μg mL-1 for method A, 7.5-37.5 μg mL-1 for method B and 5 -25 μg mL-1 for method C. For more accurate results, ringbom optimum concentration ranges are 5-18 μg mL-1 for method A , 8.5-35.5 μg mL-1 for method B and 6.0-23.0 μg mL-1 for method C. The molar absorptivities are 3.55x104, 2.10x104 and 3.10x104 L mol-1 cm-1. Where as sandell sensitivities are 0.024, 0.041 and 0.028 μg cm-22 for method A, B and C respectively. The mean percentage recoveries are 99.95 for method A, 101.35 for method B and 100.33 for method C. The developed methods were applied for the determination of PST in bulk powder and in the pharmaceutical formulations without any interference from tablet excipients. |
| format | Article |
| id | doaj-art-90ee45b91c3e4be8be7645fa5bcf136e |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-90ee45b91c3e4be8be7645fa5bcf136e2025-02-03T01:21:01ZengWileyE-Journal of Chemistry0973-49452090-98102007-01-014227227810.1155/2007/628987Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical FormulationsMarothu Vamsi Krishna0Dannana Gowri Sankar1Pharmaceutical Analysis and Quality assurance division, College of pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, IndiaPharmaceutical Analysis and Quality assurance division, College of pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, IndiaThree simple, sensitive and cost effective Spectrophotometric methods are described for the determination of pitavastatin calcium (PST) in bulk drugs and in pharmaceutical formulations. These methods are based on the oxidation of PST by ferric chloride in presence of o-phenanthroline (Method A) or 2, 2’ bipyridyl (Method B) or potassium ferricyanide (Method C). The colored complex formed was measured at 510, 530 and 755 nm for method A, B and C respectively against the reagent blank prepared in the same manner. The optimum experimental parameters for the color production are selected. Beer’s law is valid with in a concentration range of 4-20 μg mL-1 for method A, 7.5-37.5 μg mL-1 for method B and 5 -25 μg mL-1 for method C. For more accurate results, ringbom optimum concentration ranges are 5-18 μg mL-1 for method A , 8.5-35.5 μg mL-1 for method B and 6.0-23.0 μg mL-1 for method C. The molar absorptivities are 3.55x104, 2.10x104 and 3.10x104 L mol-1 cm-1. Where as sandell sensitivities are 0.024, 0.041 and 0.028 μg cm-22 for method A, B and C respectively. The mean percentage recoveries are 99.95 for method A, 101.35 for method B and 100.33 for method C. The developed methods were applied for the determination of PST in bulk powder and in the pharmaceutical formulations without any interference from tablet excipients.http://dx.doi.org/10.1155/2007/628987 |
| spellingShingle | Marothu Vamsi Krishna Dannana Gowri Sankar Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations E-Journal of Chemistry |
| title | Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations |
| title_full | Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations |
| title_fullStr | Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations |
| title_full_unstemmed | Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations |
| title_short | Adaptation of Color Reactions for Spectrophotometric Determination of Pitavastatin Calcium in Bulk Drugs and in Pharmaceutical Formulations |
| title_sort | adaptation of color reactions for spectrophotometric determination of pitavastatin calcium in bulk drugs and in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2007/628987 |
| work_keys_str_mv | AT marothuvamsikrishna adaptationofcolorreactionsforspectrophotometricdeterminationofpitavastatincalciuminbulkdrugsandinpharmaceuticalformulations AT dannanagowrisankar adaptationofcolorreactionsforspectrophotometricdeterminationofpitavastatincalciuminbulkdrugsandinpharmaceuticalformulations |