Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro
IntroductionEarly preimplantation embryos of mammals exhibit pronounced sensitivity to hyperosmotic conditions, which results in an embryonic developmental block. The reduction of medium osmolarity or the supplementation with organic osmolytes can eliminate this arrest. Therefore, cell volume homeos...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1524749/full |
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| author | Lixiang Liu Lixiang Liu Yu Tang Yu Tang Jing Shao Jing Shao Bingfeng Fan Bingfeng Fan Yifeng Yang Yifeng Yang Ying Zhang Ying Zhang Xiangyuan Zhao Xiangyuan Zhao Hailong Xue Hailong Xue Huimin Sun Huimin Sun Xulin Zhang Xulin Zhang Yushi Zhang Yushi Zhang Baozeng Xu Baozeng Xu |
| author_facet | Lixiang Liu Lixiang Liu Yu Tang Yu Tang Jing Shao Jing Shao Bingfeng Fan Bingfeng Fan Yifeng Yang Yifeng Yang Ying Zhang Ying Zhang Xiangyuan Zhao Xiangyuan Zhao Hailong Xue Hailong Xue Huimin Sun Huimin Sun Xulin Zhang Xulin Zhang Yushi Zhang Yushi Zhang Baozeng Xu Baozeng Xu |
| author_sort | Lixiang Liu |
| collection | DOAJ |
| description | IntroductionEarly preimplantation embryos of mammals exhibit pronounced sensitivity to hyperosmotic conditions, which results in an embryonic developmental block. The reduction of medium osmolarity or the supplementation with organic osmolytes can eliminate this arrest. Therefore, cell volume homeostasis is essential for embryonic development in vitro. Oocytes become capable of independent volume regulation after detaching from the follicle microenvironment. Whether the efficiency and quality of oocyte maturation can be improved by optimizing cell volume regulation by adjusting the osmolality of the culture medium in the presence of the organic osmolyte of glycine remains to be determined.MethodsThe IVM of porcine oocytes was divided into two stages, i.e. the first 22 h as the first stage, and the last 22 h as the second stage. In the presence of 1 mM glycine, we adjusted the osmolality of the culture medium from low to high (290 mOsM for the first 22 h and 320 mOsM thereafter) by adding raffinose, which cannot be used by animal cells, in a culture stage-dependent manner.ResultsStage-dependent adjustment of simplified medium PZM-3 osmolarity (290 mOsM for the first 22 h and 320 mOsM thereafter) in the presence of 1 mM glycine significantly improved the quality of porcine oocyte maturation in vitro, manifested by the oocyte maturation rate, functional mitochondrial distribution and activity, the transcript levels of glycolysis genes in granulosa cells, and subsequent embryonic developmental ability and ROS levels.ConclusionOur study demonstrates that optimizing cell volume regulation can further enhance the developmental potential of oocytes cultured in vitro. |
| format | Article |
| id | doaj-art-90dacfd5dc844f17bff9ff06b869e425 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-90dacfd5dc844f17bff9ff06b869e4252025-01-30T06:22:29ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011310.3389/fcell.2025.15247491524749Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitroLixiang Liu0Lixiang Liu1Yu Tang2Yu Tang3Jing Shao4Jing Shao5Bingfeng Fan6Bingfeng Fan7Yifeng Yang8Yifeng Yang9Ying Zhang10Ying Zhang11Xiangyuan Zhao12Xiangyuan Zhao13Hailong Xue14Hailong Xue15Huimin Sun16Huimin Sun17Xulin Zhang18Xulin Zhang19Yushi Zhang20Yushi Zhang21Baozeng Xu22Baozeng Xu23Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaState Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, ChinaIntroductionEarly preimplantation embryos of mammals exhibit pronounced sensitivity to hyperosmotic conditions, which results in an embryonic developmental block. The reduction of medium osmolarity or the supplementation with organic osmolytes can eliminate this arrest. Therefore, cell volume homeostasis is essential for embryonic development in vitro. Oocytes become capable of independent volume regulation after detaching from the follicle microenvironment. Whether the efficiency and quality of oocyte maturation can be improved by optimizing cell volume regulation by adjusting the osmolality of the culture medium in the presence of the organic osmolyte of glycine remains to be determined.MethodsThe IVM of porcine oocytes was divided into two stages, i.e. the first 22 h as the first stage, and the last 22 h as the second stage. In the presence of 1 mM glycine, we adjusted the osmolality of the culture medium from low to high (290 mOsM for the first 22 h and 320 mOsM thereafter) by adding raffinose, which cannot be used by animal cells, in a culture stage-dependent manner.ResultsStage-dependent adjustment of simplified medium PZM-3 osmolarity (290 mOsM for the first 22 h and 320 mOsM thereafter) in the presence of 1 mM glycine significantly improved the quality of porcine oocyte maturation in vitro, manifested by the oocyte maturation rate, functional mitochondrial distribution and activity, the transcript levels of glycolysis genes in granulosa cells, and subsequent embryonic developmental ability and ROS levels.ConclusionOur study demonstrates that optimizing cell volume regulation can further enhance the developmental potential of oocytes cultured in vitro.https://www.frontiersin.org/articles/10.3389/fcell.2025.1524749/fullporcine oocytescell volume regulationosmolarityglycinein vitro maturation |
| spellingShingle | Lixiang Liu Lixiang Liu Yu Tang Yu Tang Jing Shao Jing Shao Bingfeng Fan Bingfeng Fan Yifeng Yang Yifeng Yang Ying Zhang Ying Zhang Xiangyuan Zhao Xiangyuan Zhao Hailong Xue Hailong Xue Huimin Sun Huimin Sun Xulin Zhang Xulin Zhang Yushi Zhang Yushi Zhang Baozeng Xu Baozeng Xu Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro Frontiers in Cell and Developmental Biology porcine oocytes cell volume regulation osmolarity glycine in vitro maturation |
| title | Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| title_full | Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| title_fullStr | Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| title_full_unstemmed | Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| title_short | Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| title_sort | stage dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro |
| topic | porcine oocytes cell volume regulation osmolarity glycine in vitro maturation |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1524749/full |
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