HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn.
A simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the stem extract of Hibiscus micranthus Linn. A flavonoid fraction of methanolic extract of the stem powder was used for the experimental work. Separation was perf...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/902106 |
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| author | K. Ashok Kumar S. Ramachandra Shetty Laxmi Narasu |
| author_facet | K. Ashok Kumar S. Ramachandra Shetty Laxmi Narasu |
| author_sort | K. Ashok Kumar |
| collection | DOAJ |
| description | A simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the stem extract of Hibiscus micranthus Linn. A flavonoid fraction of methanolic extract of the stem powder was used for the experimental work. Separation was performed on silica gel 60 F254 HPTLC plates with n-butanol: acetic acid: water: ammonia 3:1:1:1(v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 286 nm. Rutin response was linear over the range 50–400 ng/μL. The concentration of rutin in the flavanoidal fraction was found to be 9.93%. The HPTLC method was evaluated in terms of sensitivity, accuracy, precision and reproducibility. |
| format | Article |
| id | doaj-art-90bcfab19707484fb532403b7908613a |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-90bcfab19707484fb532403b7908613a2025-02-03T01:21:10ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01831444145010.1155/2011/902106HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn.K. Ashok Kumar0S. Ramachandra Shetty1Laxmi Narasu2Department of Pharmacognosy, Bharat Institute of Technology Pharmacy, Ibrahimpatnam, RR Dist-510501, IndiaDepartment of Pharmacology, College of Clinical Pharmacy, King Faisal University, Alahsa-31982, IndiaDepartment of Biotechnology, Jawaharlal Nehru Technological University, Kukattpally, Hyderabad-517132, Andhra Pradesh, IndiaA simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the stem extract of Hibiscus micranthus Linn. A flavonoid fraction of methanolic extract of the stem powder was used for the experimental work. Separation was performed on silica gel 60 F254 HPTLC plates with n-butanol: acetic acid: water: ammonia 3:1:1:1(v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 286 nm. Rutin response was linear over the range 50–400 ng/μL. The concentration of rutin in the flavanoidal fraction was found to be 9.93%. The HPTLC method was evaluated in terms of sensitivity, accuracy, precision and reproducibility.http://dx.doi.org/10.1155/2011/902106 |
| spellingShingle | K. Ashok Kumar S. Ramachandra Shetty Laxmi Narasu HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. E-Journal of Chemistry |
| title | HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. |
| title_full | HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. |
| title_fullStr | HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. |
| title_full_unstemmed | HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. |
| title_short | HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction of Hibiscus micranthus Linn. |
| title_sort | hptlc method development and validation for determination of rutin in flavanoidal fraction of hibiscus micranthus linn |
| url | http://dx.doi.org/10.1155/2011/902106 |
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