Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells
Stem cell-based therapies rely on stem cell ability to repair in an oxidative stress environment. Preconditioning of mesenchymal stem cells (MSCs) to a stress environment has beneficial effects on their ability to repair injured tissues. We previously reported that MSCs from the decidua basalis (DBM...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/6480793 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552371140427776 |
|---|---|
| author | T. Khatlani D. Algudiri R. Alenzi A. M. Al Subayyil F. M. Abomaray E. Bahattab A. S. AlAskar B. Kalionis M. F. El-Muzaini M. H. Abumaree |
| author_facet | T. Khatlani D. Algudiri R. Alenzi A. M. Al Subayyil F. M. Abomaray E. Bahattab A. S. AlAskar B. Kalionis M. F. El-Muzaini M. H. Abumaree |
| author_sort | T. Khatlani |
| collection | DOAJ |
| description | Stem cell-based therapies rely on stem cell ability to repair in an oxidative stress environment. Preconditioning of mesenchymal stem cells (MSCs) to a stress environment has beneficial effects on their ability to repair injured tissues. We previously reported that MSCs from the decidua basalis (DBMSCs) of human placenta have many important cellular functions that make them potentially useful for cell-based therapies. Here, we studied the effect of DBMSC preconditioning to a stress environment. DBMSCs were exposed to various concentrations of hydrogen peroxide (H2O2), and their functions were then assessed. DBMSC expression of immune molecules after preconditioning was also determined. DBMSC preconditioning with H2O2 enhanced their proliferation, colonogenicity, adhesion, and migration. In addition, DBMSCs regardless of H2O2 treatment displayed antiangiogenic activity. H2O2 preconditioning also increased DBMSC expression of genes that promote cellular functions and decreased the expression of genes, which have opposite effect on their functions. Preconditioning also reduced DBMSC expression of IL-1β, but had no effects on the expression of other immune molecules that promote proliferation, adhesion, and migration. These data show that DBMSCs resist a toxic environment, which adds to their potential as a candidate stem cell type for treating various diseases in hostile environments. |
| format | Article |
| id | doaj-art-90a3771e90774e2eb6f759c6de275328 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-90a3771e90774e2eb6f759c6de2753282025-02-03T05:58:48ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/64807936480793Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal CellsT. Khatlani0D. Algudiri1R. Alenzi2A. M. Al Subayyil3F. M. Abomaray4E. Bahattab5A. S. AlAskar6B. Kalionis7M. F. El-Muzaini8M. H. Abumaree9Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaStem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaStem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaStem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaDepartment of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, 14186 Stockholm, SwedenNational Center for Stem Cell Technology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi ArabiaStem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaDepartment of Obstetrics and Gynaecology and Department of Maternal-Fetal Medicine Pregnancy Research Centre, Royal Women’s Hospital, University of Melbourne, Parkville, VIC 3052, AustraliaDepartment of Obstetrics and Gynaecology, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 3124, P.O. Box 3660, Riyadh 11481, Saudi ArabiaStem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh 11426, Saudi ArabiaStem cell-based therapies rely on stem cell ability to repair in an oxidative stress environment. Preconditioning of mesenchymal stem cells (MSCs) to a stress environment has beneficial effects on their ability to repair injured tissues. We previously reported that MSCs from the decidua basalis (DBMSCs) of human placenta have many important cellular functions that make them potentially useful for cell-based therapies. Here, we studied the effect of DBMSC preconditioning to a stress environment. DBMSCs were exposed to various concentrations of hydrogen peroxide (H2O2), and their functions were then assessed. DBMSC expression of immune molecules after preconditioning was also determined. DBMSC preconditioning with H2O2 enhanced their proliferation, colonogenicity, adhesion, and migration. In addition, DBMSCs regardless of H2O2 treatment displayed antiangiogenic activity. H2O2 preconditioning also increased DBMSC expression of genes that promote cellular functions and decreased the expression of genes, which have opposite effect on their functions. Preconditioning also reduced DBMSC expression of IL-1β, but had no effects on the expression of other immune molecules that promote proliferation, adhesion, and migration. These data show that DBMSCs resist a toxic environment, which adds to their potential as a candidate stem cell type for treating various diseases in hostile environments.http://dx.doi.org/10.1155/2018/6480793 |
| spellingShingle | T. Khatlani D. Algudiri R. Alenzi A. M. Al Subayyil F. M. Abomaray E. Bahattab A. S. AlAskar B. Kalionis M. F. El-Muzaini M. H. Abumaree Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells Stem Cells International |
| title | Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells |
| title_full | Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells |
| title_fullStr | Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells |
| title_full_unstemmed | Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells |
| title_short | Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells |
| title_sort | preconditioning by hydrogen peroxide enhances multiple properties of human decidua basalis mesenchymal stem multipotent stromal cells |
| url | http://dx.doi.org/10.1155/2018/6480793 |
| work_keys_str_mv | AT tkhatlani preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT dalgudiri preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT ralenzi preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT amalsubayyil preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT fmabomaray preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT ebahattab preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT asalaskar preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT bkalionis preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT mfelmuzaini preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells AT mhabumaree preconditioningbyhydrogenperoxideenhancesmultiplepropertiesofhumandeciduabasalismesenchymalstemmultipotentstromalcells |