Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death
Calophyllum brasiliense is a plant from the Brazilian rain forests and has been used in folk medicine for the treatment of various diseases, including leishmaniasis. This infectious disease depends on the Leishmania sp. and the host immune response. C. brasiliense antileishmanial activity is well kn...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/6148351 |
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| author | L. Domeneghetti I. G. Demarchi J. Z. Caitano R. B. Pedroso T. G. V. Silveira M. V. C. Lonardoni |
| author_facet | L. Domeneghetti I. G. Demarchi J. Z. Caitano R. B. Pedroso T. G. V. Silveira M. V. C. Lonardoni |
| author_sort | L. Domeneghetti |
| collection | DOAJ |
| description | Calophyllum brasiliense is a plant from the Brazilian rain forests and has been used in folk medicine for the treatment of various diseases, including leishmaniasis. This infectious disease depends on the Leishmania sp. and the host immune response. C. brasiliense antileishmanial activity is well known, but the effects on immune response remain to be investigated. This study showed the leishmanicidal and immunomodulatory effects of a 30 μg/mL of hydroalcoholic extract of C. brasiliense in murine macrophages before and after Leishmania (Leishmania) amazonensis infection. The semiquantitative cytokine RNA expression was determined by RT-PCR and the anti-Leishmania activity was measured by infection index (IF). Hydroalcoholic extract of C. brasiliense reduced more than 95% of IF when used before and after Leishmania infection, with 3 and 24 h of treatment (p<0.05). C. brasiliense inhibited or reduced significantly (p<0.05) the TNF-α, IL-1β, IL-18, and IL-10 mRNA expression. The antileishmanial and anti-inflammatory effects showed the potential of C. brasiliense as an alternative therapy for leishmaniasis and it must be investigated. |
| format | Article |
| id | doaj-art-908009b4fd404b169974291169291211 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-908009b4fd404b1699742911692912112025-02-03T01:10:27ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/61483516148351Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular DeathL. Domeneghetti0I. G. Demarchi1J. Z. Caitano2R. B. Pedroso3T. G. V. Silveira4M. V. C. Lonardoni5Graduate Program in Bioscience and Physiopathology, Departamento de Análises Clínicas e Biomedicina, The State University of Maringá, Colombo Avenue 5790, 87020-900 Maringá, PR, BrazilDepartamento de Análises Clínicas e Biomedicina, The State University of Maringá, Maringá, PR, BrazilGraduate Program in Bioscience and Physiopathology, Departamento de Análises Clínicas e Biomedicina, The State University of Maringá, Colombo Avenue 5790, 87020-900 Maringá, PR, BrazilPostgraduate Program in Health Sciences, Centro de Ciências da Saúde, State University of Maringá, Maringá, PR, BrazilDepartamento de Análises Clínicas e Biomedicina, The State University of Maringá, Maringá, PR, BrazilDepartamento de Análises Clínicas e Biomedicina, The State University of Maringá, Maringá, PR, BrazilCalophyllum brasiliense is a plant from the Brazilian rain forests and has been used in folk medicine for the treatment of various diseases, including leishmaniasis. This infectious disease depends on the Leishmania sp. and the host immune response. C. brasiliense antileishmanial activity is well known, but the effects on immune response remain to be investigated. This study showed the leishmanicidal and immunomodulatory effects of a 30 μg/mL of hydroalcoholic extract of C. brasiliense in murine macrophages before and after Leishmania (Leishmania) amazonensis infection. The semiquantitative cytokine RNA expression was determined by RT-PCR and the anti-Leishmania activity was measured by infection index (IF). Hydroalcoholic extract of C. brasiliense reduced more than 95% of IF when used before and after Leishmania infection, with 3 and 24 h of treatment (p<0.05). C. brasiliense inhibited or reduced significantly (p<0.05) the TNF-α, IL-1β, IL-18, and IL-10 mRNA expression. The antileishmanial and anti-inflammatory effects showed the potential of C. brasiliense as an alternative therapy for leishmaniasis and it must be investigated.http://dx.doi.org/10.1155/2018/6148351 |
| spellingShingle | L. Domeneghetti I. G. Demarchi J. Z. Caitano R. B. Pedroso T. G. V. Silveira M. V. C. Lonardoni Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death Mediators of Inflammation |
| title | Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death |
| title_full | Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death |
| title_fullStr | Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death |
| title_full_unstemmed | Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death |
| title_short | Calophyllum brasiliense Modulates the Immune Response and Promotes Leishmania amazonensis Intracellular Death |
| title_sort | calophyllum brasiliense modulates the immune response and promotes leishmania amazonensis intracellular death |
| url | http://dx.doi.org/10.1155/2018/6148351 |
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