Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru
Evaluation of resistance to antituberculosis drugs is routinely performed with genotypic or phenotypic methods; however, discordance can be seen between these different methodologies. Our objective was to identify mutations that could explain discordant results in the evaluation of susceptibility to...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/8253546 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553957733433344 |
|---|---|
| author | L. Solari D. Santos-Lazaro Z. M. Puyen |
| author_facet | L. Solari D. Santos-Lazaro Z. M. Puyen |
| author_sort | L. Solari |
| collection | DOAJ |
| description | Evaluation of resistance to antituberculosis drugs is routinely performed with genotypic or phenotypic methods; however, discordance can be seen between these different methodologies. Our objective was to identify mutations that could explain discordant results in the evaluation of susceptibility to rifampicin and isoniazid between molecular and phenotypic methods, using whole genome sequencing (WGS). Peruvian strains showing sensitive results in the GenoType MTBDRplus v2.0 test and resistant results in the proportions in the agar-plaque test for isoniazid or rifampin were selected. Discordance was confirmed by repeating both tests, and WGS was performed, using the Next Generation Sequencing methodology. Obtained sequences were aligned “through reference” (genomic mapping) using the program BWA with the algorithm “mem”, using as a reference the genome of the M. tuberculosis H37Rv strain. Discordance was confirmed in 14 strains for rifampicin and 21 for isoniazid, with 1 strain in common for both antibiotics, for a total of 34 unique strains. The most frequent mutation in the rpoB gene in the discordant strains for rifampicin was V170F. The most frequent mutations in the discordant strains for isoniazid were katG R463L, kasA G269S, and Rv1592c I322V. Several other mutations are reported. This is the first study in Latin America addressing mutations present in strains with discordant results between genotypic and phenotypic methods to rifampicin and isoniazid. These mutations could be considered as future potential targets for genotypic tests for evaluation of susceptibility to these drugs. |
| format | Article |
| id | doaj-art-9077e4d63e43408abff44f274d717025 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9077e4d63e43408abff44f274d7170252025-02-03T05:52:43ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/82535468253546Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in PeruL. Solari0D. Santos-Lazaro1Z. M. Puyen2Instituto Nacional de Salud, Lima, PeruInstituto Nacional de Salud, Lima, PeruInstituto Nacional de Salud, Lima, PeruEvaluation of resistance to antituberculosis drugs is routinely performed with genotypic or phenotypic methods; however, discordance can be seen between these different methodologies. Our objective was to identify mutations that could explain discordant results in the evaluation of susceptibility to rifampicin and isoniazid between molecular and phenotypic methods, using whole genome sequencing (WGS). Peruvian strains showing sensitive results in the GenoType MTBDRplus v2.0 test and resistant results in the proportions in the agar-plaque test for isoniazid or rifampin were selected. Discordance was confirmed by repeating both tests, and WGS was performed, using the Next Generation Sequencing methodology. Obtained sequences were aligned “through reference” (genomic mapping) using the program BWA with the algorithm “mem”, using as a reference the genome of the M. tuberculosis H37Rv strain. Discordance was confirmed in 14 strains for rifampicin and 21 for isoniazid, with 1 strain in common for both antibiotics, for a total of 34 unique strains. The most frequent mutation in the rpoB gene in the discordant strains for rifampicin was V170F. The most frequent mutations in the discordant strains for isoniazid were katG R463L, kasA G269S, and Rv1592c I322V. Several other mutations are reported. This is the first study in Latin America addressing mutations present in strains with discordant results between genotypic and phenotypic methods to rifampicin and isoniazid. These mutations could be considered as future potential targets for genotypic tests for evaluation of susceptibility to these drugs.http://dx.doi.org/10.1155/2020/8253546 |
| spellingShingle | L. Solari D. Santos-Lazaro Z. M. Puyen Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru International Journal of Microbiology |
| title | Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru |
| title_full | Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru |
| title_fullStr | Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru |
| title_full_unstemmed | Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru |
| title_short | Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru |
| title_sort | mutations in mycobacterium tuberculosis isolates with discordant results for drug susceptibility testing in peru |
| url | http://dx.doi.org/10.1155/2020/8253546 |
| work_keys_str_mv | AT lsolari mutationsinmycobacteriumtuberculosisisolateswithdiscordantresultsfordrugsusceptibilitytestinginperu AT dsantoslazaro mutationsinmycobacteriumtuberculosisisolateswithdiscordantresultsfordrugsusceptibilitytestinginperu AT zmpuyen mutationsinmycobacteriumtuberculosisisolateswithdiscordantresultsfordrugsusceptibilitytestinginperu |