Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes
South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (<i>Vicugna pacos</i>, N = 12) and the immunoexpression of antioxidant selenoproteins...
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2025-01-01
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| author | Miluska Beatriz Navarrete Zamora Francisco Acuña Mônica Duarte da Silva Thamires Santos-Silva Matheus Henrique Herminio Garcia Rodrigo da Silva Nunes Barreto Alberto Sato Sato Claudio Gustavo Barbeito Maria Angelica Miglino |
| author_facet | Miluska Beatriz Navarrete Zamora Francisco Acuña Mônica Duarte da Silva Thamires Santos-Silva Matheus Henrique Herminio Garcia Rodrigo da Silva Nunes Barreto Alberto Sato Sato Claudio Gustavo Barbeito Maria Angelica Miglino |
| author_sort | Miluska Beatriz Navarrete Zamora |
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| description | South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (<i>Vicugna pacos</i>, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3–5 µm), stained with hematoxylin–eosin (H&E), Masson’s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm<sup>2</sup>), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-9070e0cd64af43e5a4670d40a51754a02025-01-24T13:23:29ZengMDPI AGBiology2079-77372025-01-011416410.3390/biology14010064Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian AndesMiluska Beatriz Navarrete Zamora0Francisco Acuña1Mônica Duarte da Silva2Thamires Santos-Silva3Matheus Henrique Herminio Garcia4Rodrigo da Silva Nunes Barreto5Alberto Sato Sato6Claudio Gustavo Barbeito7Maria Angelica Miglino8Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15001, PeruFacultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata 1900, Buenos Aires, ArgentinaDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, São Paulo, BrazilDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, São Paulo, BrazilDepartment of Animal Anatomy, University of Marilia, Marília 17525-902, São Paulo, BrazilDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, São Paulo, BrazilFacultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15001, PeruFacultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata 1900, Buenos Aires, ArgentinaDepartment of Animal Anatomy, University of Marilia, Marília 17525-902, São Paulo, BrazilSouth American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (<i>Vicugna pacos</i>, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3–5 µm), stained with hematoxylin–eosin (H&E), Masson’s trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 ± 9.82 µm<sup>2</sup>), and type III collagen fibers. The chorionic villi measured 2740.22 ± 132.75 µm. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies.https://www.mdpi.com/2079-7737/14/1/64camelidsextreme environmentsfetusseleniumplacenta |
| spellingShingle | Miluska Beatriz Navarrete Zamora Francisco Acuña Mônica Duarte da Silva Thamires Santos-Silva Matheus Henrique Herminio Garcia Rodrigo da Silva Nunes Barreto Alberto Sato Sato Claudio Gustavo Barbeito Maria Angelica Miglino Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes Biology camelids extreme environments fetus selenium placenta |
| title | Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes |
| title_full | Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes |
| title_fullStr | Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes |
| title_full_unstemmed | Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes |
| title_short | Morphology and Immunoexpression of Selenoproteins in Term Placenta of Alpaca (<i>Vicugna pacos</i>) from the Peruvian Andes |
| title_sort | morphology and immunoexpression of selenoproteins in term placenta of alpaca i vicugna pacos i from the peruvian andes |
| topic | camelids extreme environments fetus selenium placenta |
| url | https://www.mdpi.com/2079-7737/14/1/64 |
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