Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)
The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same...
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| Format: | Article |
| Language: | English |
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2016-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2016-0028 |
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| author | Abdel-Lateef Ezzat Mahmoud Faten Hammam Olfat El-Ahwany Eman El-Wakil Eman Kandil Sherihan Abu Taleb Hoda El-Sayed Mortada Hassenein Hanaa |
| author_facet | Abdel-Lateef Ezzat Mahmoud Faten Hammam Olfat El-Ahwany Eman El-Wakil Eman Kandil Sherihan Abu Taleb Hoda El-Sayed Mortada Hassenein Hanaa |
| author_sort | Abdel-Lateef Ezzat |
| collection | DOAJ |
| description | The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes. |
| format | Article |
| id | doaj-art-9052f64d94cd40a19389d04967f129b2 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-9052f64d94cd40a19389d04967f129b22025-02-02T09:44:22ZengSciendoActa Pharmaceutica1846-95582016-09-0166338739810.1515/acph-2016-0028acph-2016-0028Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)Abdel-Lateef Ezzat0Mahmoud Faten1Hammam Olfat2El-Ahwany Eman3El-Wakil Eman4Kandil Sherihan5Abu Taleb Hoda6El-Sayed Mortada7Hassenein Hanaa8Medicinal Chemistry, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptImmunology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptPathology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptImmunology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptMedicinal Chemistry, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptImmunology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptEnvironmental Research Laboratories Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptMedicinal Chemistry, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptImmunology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, EgyptThe present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes.https://doi.org/10.1515/acph-2016-0028curcuma longa l.gc-ms analysiscurcuminanticancer activity |
| spellingShingle | Abdel-Lateef Ezzat Mahmoud Faten Hammam Olfat El-Ahwany Eman El-Wakil Eman Kandil Sherihan Abu Taleb Hoda El-Sayed Mortada Hassenein Hanaa Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) Acta Pharmaceutica curcuma longa l. gc-ms analysis curcumin anticancer activity |
| title | Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) |
| title_full | Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) |
| title_fullStr | Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) |
| title_full_unstemmed | Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) |
| title_short | Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2) |
| title_sort | bioactive chemical constituents of curcuma longa l rhizomes extract inhibit the growth of human hepatoma cell line hepg2 |
| topic | curcuma longa l. gc-ms analysis curcumin anticancer activity |
| url | https://doi.org/10.1515/acph-2016-0028 |
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