Viral communities associated with human pericardial fluids in idiopathic pericarditis.
Pericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093367&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849720278950608896 |
|---|---|
| author | Laura Fancello Sonia Monteil Nikolay Popgeorgiev Romain Rivet Frédérique Gouriet Pierre-Edouard Fournier Didier Raoult Christelle Desnues |
| author_facet | Laura Fancello Sonia Monteil Nikolay Popgeorgiev Romain Rivet Frédérique Gouriet Pierre-Edouard Fournier Didier Raoult Christelle Desnues |
| author_sort | Laura Fancello |
| collection | DOAJ |
| description | Pericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and sequence-independent approach to investigate the viral DNA communities present in human pericardial fluids. Seven viral metagenomes were generated from the pericardial fluid of patients affected by pericarditis of unknown etiology and one metagenome was generated from the pericardial fluid of a sudden infant death case. As a positive control we generated one metagenome from the pericardial fluid of a patient affected by pericarditis caused by herpesvirus type 3. Furthermore, we used as negative controls a total of 6 pericardial fluids from 6 different individuals affected by pericarditis of non-infectious origin: 5 of them were sequenced as a unique pool and the remaining one was sequenced separately. The results showed a significant presence of torque teno viruses especially in one patient, while herpesviruses and papillomaviruses were present in the positive control. Co-infections by different genotypes of the same viral type (torque teno viruses) or different viruses (herpesviruses and papillomaviruses) were observed. Sequences related to bacteriophages infecting Staphylococcus, Enterobacteria, Streptococcus, Burkholderia and Pseudomonas were also detected in three patients. This study detected torque teno viruses and papillomaviruses, for the first time, in human pericardial fluids. |
| format | Article |
| id | doaj-art-904a61cfe4e4475ab9fcd0aedabca20e |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-904a61cfe4e4475ab9fcd0aedabca20e2025-08-20T03:11:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9336710.1371/journal.pone.0093367Viral communities associated with human pericardial fluids in idiopathic pericarditis.Laura FancelloSonia MonteilNikolay PopgeorgievRomain RivetFrédérique GourietPierre-Edouard FournierDidier RaoultChristelle DesnuesPericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and sequence-independent approach to investigate the viral DNA communities present in human pericardial fluids. Seven viral metagenomes were generated from the pericardial fluid of patients affected by pericarditis of unknown etiology and one metagenome was generated from the pericardial fluid of a sudden infant death case. As a positive control we generated one metagenome from the pericardial fluid of a patient affected by pericarditis caused by herpesvirus type 3. Furthermore, we used as negative controls a total of 6 pericardial fluids from 6 different individuals affected by pericarditis of non-infectious origin: 5 of them were sequenced as a unique pool and the remaining one was sequenced separately. The results showed a significant presence of torque teno viruses especially in one patient, while herpesviruses and papillomaviruses were present in the positive control. Co-infections by different genotypes of the same viral type (torque teno viruses) or different viruses (herpesviruses and papillomaviruses) were observed. Sequences related to bacteriophages infecting Staphylococcus, Enterobacteria, Streptococcus, Burkholderia and Pseudomonas were also detected in three patients. This study detected torque teno viruses and papillomaviruses, for the first time, in human pericardial fluids.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093367&type=printable |
| spellingShingle | Laura Fancello Sonia Monteil Nikolay Popgeorgiev Romain Rivet Frédérique Gouriet Pierre-Edouard Fournier Didier Raoult Christelle Desnues Viral communities associated with human pericardial fluids in idiopathic pericarditis. PLoS ONE |
| title | Viral communities associated with human pericardial fluids in idiopathic pericarditis. |
| title_full | Viral communities associated with human pericardial fluids in idiopathic pericarditis. |
| title_fullStr | Viral communities associated with human pericardial fluids in idiopathic pericarditis. |
| title_full_unstemmed | Viral communities associated with human pericardial fluids in idiopathic pericarditis. |
| title_short | Viral communities associated with human pericardial fluids in idiopathic pericarditis. |
| title_sort | viral communities associated with human pericardial fluids in idiopathic pericarditis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093367&type=printable |
| work_keys_str_mv | AT laurafancello viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT soniamonteil viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT nikolaypopgeorgiev viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT romainrivet viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT frederiquegouriet viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT pierreedouardfournier viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT didierraoult viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis AT christelledesnues viralcommunitiesassociatedwithhumanpericardialfluidsinidiopathicpericarditis |