Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine
COVID-19 has caused widespread morbidity and mortality, with its effects extending to multiple organ systems. Despite known risk factors for severe disease, including advanced age and underlying comorbidities, patient outcomes can vary significantly. This variability complicates efforts to predict d...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/2075-4418/15/2/229 |
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| author | Heba M. Saad Eldien Abdulrahman H. Almaeen Ahmed Abo El Fath Ahmed E. Taha Rehab Ahmed Hassabelrasoul Elfadil Helal F. Hetta |
| author_facet | Heba M. Saad Eldien Abdulrahman H. Almaeen Ahmed Abo El Fath Ahmed E. Taha Rehab Ahmed Hassabelrasoul Elfadil Helal F. Hetta |
| author_sort | Heba M. Saad Eldien |
| collection | DOAJ |
| description | COVID-19 has caused widespread morbidity and mortality, with its effects extending to multiple organ systems. Despite known risk factors for severe disease, including advanced age and underlying comorbidities, patient outcomes can vary significantly. This variability complicates efforts to predict disease progression and tailor treatment strategies. While diagnostic and therapeutic approaches are still under debate, RNA sequencing (RNAseq) has emerged as a promising tool to provide deeper insights into the pathophysiology of COVID-19 and guide personalized treatment. A comprehensive literature review was conducted using PubMed, Scopus, Web of Science, and Google Scholar. We employed Medical Subject Headings (MeSH) terms and relevant keywords to identify studies that explored the role of RNAseq in COVID-19 diagnostics, prognostics, and therapeutics. RNAseq has proven instrumental in identifying molecular biomarkers associated with disease severity in patients with COVID-19. It allows for the differentiation between asymptomatic and symptomatic individuals and sheds light on the immune response mechanisms that contribute to disease progression. In critically ill patients, RNAseq has been crucial for identifying key genes that may predict patient outcomes, guiding therapeutic decisions, and assessing the long-term effects of the virus. Additionally, RNAseq has helped in understanding the persistence of viral RNA after recovery, offering new insights into the management of post-acute sequelae, including long COVID. RNA sequencing significantly improves COVID-19 management, particularly for critically ill patients, by enhancing diagnostic accuracy, personalizing treatment, and predicting therapeutic responses. It refines patient stratification, improving outcomes, and holds promise for targeted interventions in both acute and long COVID. |
| format | Article |
| id | doaj-art-903a43d1281d41b4ae5d9af40e15ce17 |
| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-903a43d1281d41b4ae5d9af40e15ce172025-01-24T13:29:11ZengMDPI AGDiagnostics2075-44182025-01-0115222910.3390/diagnostics15020229Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized MedicineHeba M. Saad Eldien0Abdulrahman H. Almaeen1Ahmed Abo El Fath2Ahmed E. Taha3Rehab Ahmed4Hassabelrasoul Elfadil5Helal F. Hetta6Department of Anatomy, College of Medicine, Jouf University, Sakaka 72388, Saudi ArabiaDepartment of Pathology, College of Medicine, Jouf University, Sakaka 72388, Saudi ArabiaTropical Medicine and Gastroenterology Department, Assiut University Hospital, Assiut 71515, EgyptMicrobiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka 72388, Saudi ArabiaDivision of Microbiology, Immunology and Biotechnology, Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaDivision of Microbiology, Immunology and Biotechnology, Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaDivision of Microbiology, Immunology and Biotechnology, Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi ArabiaCOVID-19 has caused widespread morbidity and mortality, with its effects extending to multiple organ systems. Despite known risk factors for severe disease, including advanced age and underlying comorbidities, patient outcomes can vary significantly. This variability complicates efforts to predict disease progression and tailor treatment strategies. While diagnostic and therapeutic approaches are still under debate, RNA sequencing (RNAseq) has emerged as a promising tool to provide deeper insights into the pathophysiology of COVID-19 and guide personalized treatment. A comprehensive literature review was conducted using PubMed, Scopus, Web of Science, and Google Scholar. We employed Medical Subject Headings (MeSH) terms and relevant keywords to identify studies that explored the role of RNAseq in COVID-19 diagnostics, prognostics, and therapeutics. RNAseq has proven instrumental in identifying molecular biomarkers associated with disease severity in patients with COVID-19. It allows for the differentiation between asymptomatic and symptomatic individuals and sheds light on the immune response mechanisms that contribute to disease progression. In critically ill patients, RNAseq has been crucial for identifying key genes that may predict patient outcomes, guiding therapeutic decisions, and assessing the long-term effects of the virus. Additionally, RNAseq has helped in understanding the persistence of viral RNA after recovery, offering new insights into the management of post-acute sequelae, including long COVID. RNA sequencing significantly improves COVID-19 management, particularly for critically ill patients, by enhancing diagnostic accuracy, personalizing treatment, and predicting therapeutic responses. It refines patient stratification, improving outcomes, and holds promise for targeted interventions in both acute and long COVID.https://www.mdpi.com/2075-4418/15/2/229COVID-19RNAseqtranscriptomehost responsesgeneprecision medicine |
| spellingShingle | Heba M. Saad Eldien Abdulrahman H. Almaeen Ahmed Abo El Fath Ahmed E. Taha Rehab Ahmed Hassabelrasoul Elfadil Helal F. Hetta Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine Diagnostics COVID-19 RNAseq transcriptome host responses gene precision medicine |
| title | Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine |
| title_full | Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine |
| title_fullStr | Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine |
| title_full_unstemmed | Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine |
| title_short | Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine |
| title_sort | unlocking the potential of rna sequencing in covid 19 toward accurate diagnosis and personalized medicine |
| topic | COVID-19 RNAseq transcriptome host responses gene precision medicine |
| url | https://www.mdpi.com/2075-4418/15/2/229 |
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