Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples
Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/7495218 |
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| author | Munmun B. Marak Biranthabail Dhanashree |
| author_facet | Munmun B. Marak Biranthabail Dhanashree |
| author_sort | Munmun B. Marak |
| collection | DOAJ |
| description | Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient’s demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment. |
| format | Article |
| id | doaj-art-9001d17b500a454494e427186c115f66 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9001d17b500a454494e427186c115f662025-02-03T01:26:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/74952187495218Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical SamplesMunmun B. Marak0Biranthabail Dhanashree1Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, IndiaDepartment of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, IndiaObjective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient’s demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.http://dx.doi.org/10.1155/2018/7495218 |
| spellingShingle | Munmun B. Marak Biranthabail Dhanashree Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples International Journal of Microbiology |
| title | Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples |
| title_full | Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples |
| title_fullStr | Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples |
| title_full_unstemmed | Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples |
| title_short | Antifungal Susceptibility and Biofilm Production of Candida spp. Isolated from Clinical Samples |
| title_sort | antifungal susceptibility and biofilm production of candida spp isolated from clinical samples |
| url | http://dx.doi.org/10.1155/2018/7495218 |
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