Fecal Microbiota and Diet of Children with Chronic Constipation
Many factors explain dysbiosis in chronic constipation (CC), such as a low-fiber diet. The objective of this study was to compare the fecal microbiota of constipated and nonconstipated children and their intake frequencies of food. Methods. This observational study included 79 children (M/F 43/36) a...
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| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Pediatrics |
| Online Access: | http://dx.doi.org/10.1155/2016/6787269 |
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| author | Joyce Gomes de Moraes Maria Eugênia Farias de Almeida Motta Monique Ferraz de Sá Beltrão Taciana Lima Salviano Giselia Alves Pontes da Silva |
| author_facet | Joyce Gomes de Moraes Maria Eugênia Farias de Almeida Motta Monique Ferraz de Sá Beltrão Taciana Lima Salviano Giselia Alves Pontes da Silva |
| author_sort | Joyce Gomes de Moraes |
| collection | DOAJ |
| description | Many factors explain dysbiosis in chronic constipation (CC), such as a low-fiber diet. The objective of this study was to compare the fecal microbiota of constipated and nonconstipated children and their intake frequencies of food. Methods. This observational study included 79 children (M/F 43/36) aged six to 36 months divided into two groups: cases (39 constipated children) and controls (40 nonconstipated children). We used a structured form to collect demographic variables, conducted anthropometric assessment, and collected food intake frequency data. The fecal microbiota of the stool samples was analyzed by real-time polymerase chain reaction (PCR) using the fluorophore SYBR® Green. Results. Constipated children had a smaller concentration of Lactobacillus per milligram of stool (p=0.015) than nonconstipated children, but the concentration of Bifidobacterium per milligram of stool (p=0.323) and the intake of fruits, vegetables (p=0.563), and junk food (p=0.093) of the two groups did not differ. Constipated children consumed more dairy products (0.45±0.8; p>0.001), were more frequently delivered via caesarean section (69.2%), were weaned earlier (median: 120; 60Q1–240Q3), and had a family history of constipation (71.8%). Conclusions. Children with CC have a smaller concentration of Lactobacillus in their stools and consume more dairy products. |
| format | Article |
| id | doaj-art-8ff550ed73ba4d66aaea707ef32412fc |
| institution | Kabale University |
| issn | 1687-9740 1687-9759 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Pediatrics |
| spelling | doaj-art-8ff550ed73ba4d66aaea707ef32412fc2025-02-03T01:07:46ZengWileyInternational Journal of Pediatrics1687-97401687-97592016-01-01201610.1155/2016/67872696787269Fecal Microbiota and Diet of Children with Chronic ConstipationJoyce Gomes de Moraes0Maria Eugênia Farias de Almeida Motta1Monique Ferraz de Sá Beltrão2Taciana Lima Salviano3Giselia Alves Pontes da Silva4Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, BrazilMother-Child Department, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, BrazilDepartment of Biotechnology, Laboratory of Immunopathology Keizo Asami (LIKA), Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, BrazilDepartment of Biotechnology, Laboratory of Immunopathology Keizo Asami (LIKA), Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, BrazilMother-Child Department, Federal University of Pernambuco, Avenida Professor Moraes Rego 1235, University City, 50670-901 Recife, PE, BrazilMany factors explain dysbiosis in chronic constipation (CC), such as a low-fiber diet. The objective of this study was to compare the fecal microbiota of constipated and nonconstipated children and their intake frequencies of food. Methods. This observational study included 79 children (M/F 43/36) aged six to 36 months divided into two groups: cases (39 constipated children) and controls (40 nonconstipated children). We used a structured form to collect demographic variables, conducted anthropometric assessment, and collected food intake frequency data. The fecal microbiota of the stool samples was analyzed by real-time polymerase chain reaction (PCR) using the fluorophore SYBR® Green. Results. Constipated children had a smaller concentration of Lactobacillus per milligram of stool (p=0.015) than nonconstipated children, but the concentration of Bifidobacterium per milligram of stool (p=0.323) and the intake of fruits, vegetables (p=0.563), and junk food (p=0.093) of the two groups did not differ. Constipated children consumed more dairy products (0.45±0.8; p>0.001), were more frequently delivered via caesarean section (69.2%), were weaned earlier (median: 120; 60Q1–240Q3), and had a family history of constipation (71.8%). Conclusions. Children with CC have a smaller concentration of Lactobacillus in their stools and consume more dairy products.http://dx.doi.org/10.1155/2016/6787269 |
| spellingShingle | Joyce Gomes de Moraes Maria Eugênia Farias de Almeida Motta Monique Ferraz de Sá Beltrão Taciana Lima Salviano Giselia Alves Pontes da Silva Fecal Microbiota and Diet of Children with Chronic Constipation International Journal of Pediatrics |
| title | Fecal Microbiota and Diet of Children with Chronic Constipation |
| title_full | Fecal Microbiota and Diet of Children with Chronic Constipation |
| title_fullStr | Fecal Microbiota and Diet of Children with Chronic Constipation |
| title_full_unstemmed | Fecal Microbiota and Diet of Children with Chronic Constipation |
| title_short | Fecal Microbiota and Diet of Children with Chronic Constipation |
| title_sort | fecal microbiota and diet of children with chronic constipation |
| url | http://dx.doi.org/10.1155/2016/6787269 |
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