Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents
Irisin is a cleaved and secreted fragment of fibronectin type III domain containing 5 (FNDC5) that is mainly released by skeletal muscle and was proposed to mediate the beneficial effects of exercise on metabolism. In the present study we aim to investigate the regulation of the circulating levels o...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/620919 |
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| author | Mar Quiñones Cintia Folgueira Estrella Sánchez-Rebordelo Omar Al-Massadi |
| author_facet | Mar Quiñones Cintia Folgueira Estrella Sánchez-Rebordelo Omar Al-Massadi |
| author_sort | Mar Quiñones |
| collection | DOAJ |
| description | Irisin is a cleaved and secreted fragment of fibronectin type III domain containing 5 (FNDC5) that is mainly released by skeletal muscle and was proposed to mediate the beneficial effects of exercise on metabolism. In the present study we aim to investigate the regulation of the circulating levels of irisin in obese animal models (diet-induced obese (DIO) rats and leptin-deficient (ob/ob) mice), as well as the influence of nutritional status and leptin. Irisin levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA). Serum irisin levels remained unaltered in DIO rats and ob/ob mice. Moreover, its circulating levels were also unaffected by fasting, leptin deficiency, and exogenous leptin administration in rodents. In spite of these negative results we find a negative correlation between irisin and insulin in DIO animals and a positive correlation between irisin and glucose under short-term changes in nutritional status. Our findings indicate that serum irisin levels are not modulated by different physiological settings associated to alterations in energy homeostasis. These results suggest that in rodents circulating levels of irisin are not involved in the pathophysiology of obesity and could be unrelated to metabolic status; however, further studies should clarify its precise role in states of glucose homeostasis imbalance. |
| format | Article |
| id | doaj-art-8fe20f64cec44c13b630eb86904f42d0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8fe20f64cec44c13b630eb86904f42d02025-02-03T06:48:16ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/620919620919Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in RodentsMar Quiñones0Cintia Folgueira1Estrella Sánchez-Rebordelo2Omar Al-Massadi3Department of Physiology, CIMUS, University of Santiago de Compostela, Sanitary Research Institute of Santiago de Compostela (IDIS), 15782 Santiago de Compostela, SpainDepartment of Physiology, CIMUS, University of Santiago de Compostela, Sanitary Research Institute of Santiago de Compostela (IDIS), 15782 Santiago de Compostela, SpainDepartment of Physiology, CIMUS, University of Santiago de Compostela, Sanitary Research Institute of Santiago de Compostela (IDIS), 15782 Santiago de Compostela, SpainDepartment of Physiology, CIMUS, University of Santiago de Compostela, Sanitary Research Institute of Santiago de Compostela (IDIS), 15782 Santiago de Compostela, SpainIrisin is a cleaved and secreted fragment of fibronectin type III domain containing 5 (FNDC5) that is mainly released by skeletal muscle and was proposed to mediate the beneficial effects of exercise on metabolism. In the present study we aim to investigate the regulation of the circulating levels of irisin in obese animal models (diet-induced obese (DIO) rats and leptin-deficient (ob/ob) mice), as well as the influence of nutritional status and leptin. Irisin levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA). Serum irisin levels remained unaltered in DIO rats and ob/ob mice. Moreover, its circulating levels were also unaffected by fasting, leptin deficiency, and exogenous leptin administration in rodents. In spite of these negative results we find a negative correlation between irisin and insulin in DIO animals and a positive correlation between irisin and glucose under short-term changes in nutritional status. Our findings indicate that serum irisin levels are not modulated by different physiological settings associated to alterations in energy homeostasis. These results suggest that in rodents circulating levels of irisin are not involved in the pathophysiology of obesity and could be unrelated to metabolic status; however, further studies should clarify its precise role in states of glucose homeostasis imbalance.http://dx.doi.org/10.1155/2015/620919 |
| spellingShingle | Mar Quiñones Cintia Folgueira Estrella Sánchez-Rebordelo Omar Al-Massadi Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents Mediators of Inflammation |
| title | Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents |
| title_full | Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents |
| title_fullStr | Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents |
| title_full_unstemmed | Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents |
| title_short | Circulating Irisin Levels Are Not Regulated by Nutritional Status, Obesity, or Leptin Levels in Rodents |
| title_sort | circulating irisin levels are not regulated by nutritional status obesity or leptin levels in rodents |
| url | http://dx.doi.org/10.1155/2015/620919 |
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