Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration
Cell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt is a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective of this study was to investigate the migratory response of MSCs to a developed cobalt-incorporated hydroxyapati...
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MDPI AG
2025-02-01
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| Series: | Journal of Functional Biomaterials |
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| author | Weerapat Leelasangsai Krongrat Thummachot Puttita Thammasarnsophon Autcharaporn Srion Jintamai Suwanprateeb Somying Patntirapong |
| author_facet | Weerapat Leelasangsai Krongrat Thummachot Puttita Thammasarnsophon Autcharaporn Srion Jintamai Suwanprateeb Somying Patntirapong |
| author_sort | Weerapat Leelasangsai |
| collection | DOAJ |
| description | Cell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt is a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective of this study was to investigate the migratory response of MSCs to a developed cobalt-incorporated hydroxyapatite (HACo) material. HACo was fabricated by a simple ion exchange procedure at concentrations ranging from 40 to 8000 μM into disc shape. HACo discs were incubated in the media and conditioned media (CM; HACo<sub>CM</sub>) were collected for MSC culture. HA<sub>CM</sub> served as a control. MSCs were cultured until reaching 90% confluence before the wound was generated by scraping. Time-lapse imaging of wound migration was monitored, recorded, and assessed. Statistical analysis was performed by one-way ANOVA followed by a Dunnett’s test. The wound area gradually declined from 0 to 40 h for all samples. HACo<sub>CM</sub> at 40 µM (HACo40<sub>CM</sub>) promoted wound closure at the early period of wound healing. Both HACo40<sub>CM</sub> and HACo8000<sub>CM</sub> enhanced the distance and velocity of individual cell migration. However, only HACo40<sub>CM</sub> affected cell persistence and direction at the early period of cell migration. Exposure to HACo<sub>CM</sub> accelerated the speed of MSC migration, which is necessary for wound healing. The migratory ability of individual cells could help the rate of wound healing. Therefore, HACo materials may serve as potential biomaterials for enhanced bone healing. |
| format | Article |
| id | doaj-art-8fdb0a00a8a84fd985af84defc1cd429 |
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| issn | 2079-4983 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Biomaterials |
| spelling | doaj-art-8fdb0a00a8a84fd985af84defc1cd4292025-08-20T01:48:56ZengMDPI AGJournal of Functional Biomaterials2079-49832025-02-011637210.3390/jfb16030072Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell MigrationWeerapat Leelasangsai0Krongrat Thummachot1Puttita Thammasarnsophon2Autcharaporn Srion3Jintamai Suwanprateeb4Somying Patntirapong5Department of Implantology, Faculty of Dentistry, Thammasat University, Pathum Thani 12120, ThailandFaculty of Dentistry, Thammasat University, Pathum Thani 12120, ThailandFaculty of Dentistry, Thammasat University, Pathum Thani 12120, ThailandBiofunctional Materials and Devices Research Group, National Metal and Materials Technology Center (MTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, ThailandBiofunctional Materials and Devices Research Group, National Metal and Materials Technology Center (MTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, ThailandFaculty of Dentistry, Thammasat University, Pathum Thani 12120, ThailandCell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt is a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective of this study was to investigate the migratory response of MSCs to a developed cobalt-incorporated hydroxyapatite (HACo) material. HACo was fabricated by a simple ion exchange procedure at concentrations ranging from 40 to 8000 μM into disc shape. HACo discs were incubated in the media and conditioned media (CM; HACo<sub>CM</sub>) were collected for MSC culture. HA<sub>CM</sub> served as a control. MSCs were cultured until reaching 90% confluence before the wound was generated by scraping. Time-lapse imaging of wound migration was monitored, recorded, and assessed. Statistical analysis was performed by one-way ANOVA followed by a Dunnett’s test. The wound area gradually declined from 0 to 40 h for all samples. HACo<sub>CM</sub> at 40 µM (HACo40<sub>CM</sub>) promoted wound closure at the early period of wound healing. Both HACo40<sub>CM</sub> and HACo8000<sub>CM</sub> enhanced the distance and velocity of individual cell migration. However, only HACo40<sub>CM</sub> affected cell persistence and direction at the early period of cell migration. Exposure to HACo<sub>CM</sub> accelerated the speed of MSC migration, which is necessary for wound healing. The migratory ability of individual cells could help the rate of wound healing. Therefore, HACo materials may serve as potential biomaterials for enhanced bone healing.https://www.mdpi.com/2079-4983/16/3/72cobalthydroxyapatitewound healingcell migrationmesenchymal stem cell |
| spellingShingle | Weerapat Leelasangsai Krongrat Thummachot Puttita Thammasarnsophon Autcharaporn Srion Jintamai Suwanprateeb Somying Patntirapong Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration Journal of Functional Biomaterials cobalt hydroxyapatite wound healing cell migration mesenchymal stem cell |
| title | Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration |
| title_full | Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration |
| title_fullStr | Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration |
| title_full_unstemmed | Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration |
| title_short | Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration |
| title_sort | cobalt incorporated hydroxyapatite conditioned media promotes in vitro scratch wound healing and mesenchymal stem cell migration |
| topic | cobalt hydroxyapatite wound healing cell migration mesenchymal stem cell |
| url | https://www.mdpi.com/2079-4983/16/3/72 |
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