Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus
Dengue virus (DENV) is a major public health threat in the tropical and subtropical regions of the world. Climate change resulting from global warming is further expanding DENV–endemic areas, adversely affecting public life and health. Despite this, no specific drug against DENV has been developed s...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/3/140 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850089477922357248 |
|---|---|
| author | Jiazheng Guo Jiansheng Lu Peng Du Kexuan Cheng Chao Lei Yujia Jiang Meiling Peng Yating Li Kaiyue Sun Changyan Xu Yunzhou Yu Chen Gao Qinglin Kang Yixiao Zhang Rong Wang Zhixin Yang |
| author_facet | Jiazheng Guo Jiansheng Lu Peng Du Kexuan Cheng Chao Lei Yujia Jiang Meiling Peng Yating Li Kaiyue Sun Changyan Xu Yunzhou Yu Chen Gao Qinglin Kang Yixiao Zhang Rong Wang Zhixin Yang |
| author_sort | Jiazheng Guo |
| collection | DOAJ |
| description | Dengue virus (DENV) is a major public health threat in the tropical and subtropical regions of the world. Climate change resulting from global warming is further expanding DENV–endemic areas, adversely affecting public life and health. Despite this, no specific drug against DENV has been developed so far. Vaccines and neutralizing antibodies are the chief preventive and therapeutic tools for managing pathogenic infections. The present study describes the development of a novel fluorescence reduction neutralization test (FRNT) for evaluating the neutralizing activity of antibodies against DENV. This FRNT allows rapid antibody screening. In addition, we calculated the FRNT<sub>50</sub> to indicate the neutralizing ability of the antibodies. In contrast to the conventional plaque reduction neutralization assay, the FRNT has a shorter experimental cycle, a simpler operation, and greater objectivity, which can greatly accelerate the research and development process of vaccines and antibodies against DENV. |
| format | Article |
| id | doaj-art-8fd73742b09e49cda4d6c3058b87c1e9 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-8fd73742b09e49cda4d6c3058b87c1e92025-08-20T02:42:46ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-02-0147314010.3390/cimb47030140Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue VirusJiazheng Guo0Jiansheng Lu1Peng Du2Kexuan Cheng3Chao Lei4Yujia Jiang5Meiling Peng6Yating Li7Kaiyue Sun8Changyan Xu9Yunzhou Yu10Chen Gao11Qinglin Kang12Yixiao Zhang13Rong Wang14Zhixin Yang15Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaLaboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, ChinaDengue virus (DENV) is a major public health threat in the tropical and subtropical regions of the world. Climate change resulting from global warming is further expanding DENV–endemic areas, adversely affecting public life and health. Despite this, no specific drug against DENV has been developed so far. Vaccines and neutralizing antibodies are the chief preventive and therapeutic tools for managing pathogenic infections. The present study describes the development of a novel fluorescence reduction neutralization test (FRNT) for evaluating the neutralizing activity of antibodies against DENV. This FRNT allows rapid antibody screening. In addition, we calculated the FRNT<sub>50</sub> to indicate the neutralizing ability of the antibodies. In contrast to the conventional plaque reduction neutralization assay, the FRNT has a shorter experimental cycle, a simpler operation, and greater objectivity, which can greatly accelerate the research and development process of vaccines and antibodies against DENV.https://www.mdpi.com/1467-3045/47/3/140DENVantibodyrapid screening platformneutralization activity assayFRNT<sub>50</sub> |
| spellingShingle | Jiazheng Guo Jiansheng Lu Peng Du Kexuan Cheng Chao Lei Yujia Jiang Meiling Peng Yating Li Kaiyue Sun Changyan Xu Yunzhou Yu Chen Gao Qinglin Kang Yixiao Zhang Rong Wang Zhixin Yang Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus Current Issues in Molecular Biology DENV antibody rapid screening platform neutralization activity assay FRNT<sub>50</sub> |
| title | Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus |
| title_full | Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus |
| title_fullStr | Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus |
| title_full_unstemmed | Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus |
| title_short | Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus |
| title_sort | fluorescence reduction neutralization test a novel rapid and efficient method for characterizing the neutralizing activity of antibodies against dengue virus |
| topic | DENV antibody rapid screening platform neutralization activity assay FRNT<sub>50</sub> |
| url | https://www.mdpi.com/1467-3045/47/3/140 |
| work_keys_str_mv | AT jiazhengguo fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT jianshenglu fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT pengdu fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT kexuancheng fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT chaolei fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT yujiajiang fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT meilingpeng fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT yatingli fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT kaiyuesun fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT changyanxu fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT yunzhouyu fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT chengao fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT qinglinkang fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT yixiaozhang fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT rongwang fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus AT zhixinyang fluorescencereductionneutralizationtestanovelrapidandefficientmethodforcharacterizingtheneutralizingactivityofantibodiesagainstdenguevirus |