Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision
<b>Background/Objectives:</b> Oral cancer ranks among the top ten cancers globally, with a five-year survival rate below 50%. This study aimed to evaluate the effectiveness of autofluorescence-guided surgery compared to standard surgical methods in identifying tumor-free margins and ensu...
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MDPI AG
2024-12-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/13/1/10 |
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| author | Antonis Perdiou Ramona Dumitrescu Daniela Jumanca Octavia Balean Ruxandra Sava-Rosianu Serban Talpos Dacian Virgil Lalescu Atena Galuscan |
| author_facet | Antonis Perdiou Ramona Dumitrescu Daniela Jumanca Octavia Balean Ruxandra Sava-Rosianu Serban Talpos Dacian Virgil Lalescu Atena Galuscan |
| author_sort | Antonis Perdiou |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Oral cancer ranks among the top ten cancers globally, with a five-year survival rate below 50%. This study aimed to evaluate the effectiveness of autofluorescence-guided surgery compared to standard surgical methods in identifying tumor-free margins and ensuring complete excision. <b>Methods:</b> A prospective cohort of 80 patients was randomized into two groups: the control group underwent excision with a 10 mm margin based on clinical judgment, while the experimental group used autofluorescence guidance with a 5 mm margin beyond fluorescence visualization loss. Autofluorescence imaging was performed using the OralID device, which employs a 405 nm excitation laser to detect abnormal tissue. Ethical approval was obtained from the “Spitalul Clinic Municipal de Urgență Timișoara” Ethics Committee (approval number 08/26.02.2021), and the trial was registered at the University of Medicine and Pharmacy Timisoara (trial no. 59/25.11.2021). A double analysis was conducted: a primary analysis of the full cohort and a subgroup analysis focusing on squamous cell carcinoma (control: <i>n</i> = 19; experimental: <i>n</i> = 24). Histopathological analysis was the gold standard for margin evaluation, with margins coded as tumor-free margins (0), close (1), or infiltrated (2). <b>Results:</b> Statistically significant differences were observed in tumor-free margins between the control (73.17%) and experimental (97%) groups (<i>p</i> = 0.003). Subgroup analysis for SCC showed no significant difference (control: 84.21%; experimental: 95.83%; <i>p</i> = 0.306). Tumor location also differed significantly <i>(p</i> = 0.011), while other baseline variables, such as tumor type and patient characteristics, showed no significant differences. <b>Conclusions:</b> Autofluorescence-guided surgery improves the detection of tumor-free margins and may serve as an effective adjunct in oral cancer management. Larger studies are recommended to confirm these findings. |
| format | Article |
| id | doaj-art-8fd7281426a749afbe1c0dd6d90af386 |
| institution | Kabale University |
| issn | 2304-6767 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Dentistry Journal |
| spelling | doaj-art-8fd7281426a749afbe1c0dd6d90af3862025-01-24T13:28:35ZengMDPI AGDentistry Journal2304-67672024-12-011311010.3390/dj13010010Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor ExcisionAntonis Perdiou0Ramona Dumitrescu1Daniela Jumanca2Octavia Balean3Ruxandra Sava-Rosianu4Serban Talpos5Dacian Virgil Lalescu6Atena Galuscan7Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaFaculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaFaculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaFaculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaFaculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaDiscipline of Oral and Maxillo-Facial Surgery, Faculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaDepartment of Food Science, Faculty of Food Engineering, University of Life Sciences “King Mihai I” from Timisoara, 119 Calea Aradului Street, 300645 Timisoara, RomaniaFaculty of Dental Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania<b>Background/Objectives:</b> Oral cancer ranks among the top ten cancers globally, with a five-year survival rate below 50%. This study aimed to evaluate the effectiveness of autofluorescence-guided surgery compared to standard surgical methods in identifying tumor-free margins and ensuring complete excision. <b>Methods:</b> A prospective cohort of 80 patients was randomized into two groups: the control group underwent excision with a 10 mm margin based on clinical judgment, while the experimental group used autofluorescence guidance with a 5 mm margin beyond fluorescence visualization loss. Autofluorescence imaging was performed using the OralID device, which employs a 405 nm excitation laser to detect abnormal tissue. Ethical approval was obtained from the “Spitalul Clinic Municipal de Urgență Timișoara” Ethics Committee (approval number 08/26.02.2021), and the trial was registered at the University of Medicine and Pharmacy Timisoara (trial no. 59/25.11.2021). A double analysis was conducted: a primary analysis of the full cohort and a subgroup analysis focusing on squamous cell carcinoma (control: <i>n</i> = 19; experimental: <i>n</i> = 24). Histopathological analysis was the gold standard for margin evaluation, with margins coded as tumor-free margins (0), close (1), or infiltrated (2). <b>Results:</b> Statistically significant differences were observed in tumor-free margins between the control (73.17%) and experimental (97%) groups (<i>p</i> = 0.003). Subgroup analysis for SCC showed no significant difference (control: 84.21%; experimental: 95.83%; <i>p</i> = 0.306). Tumor location also differed significantly <i>(p</i> = 0.011), while other baseline variables, such as tumor type and patient characteristics, showed no significant differences. <b>Conclusions:</b> Autofluorescence-guided surgery improves the detection of tumor-free margins and may serve as an effective adjunct in oral cancer management. Larger studies are recommended to confirm these findings.https://www.mdpi.com/2304-6767/13/1/10autofluorescenceoral cancerpreventionmargins of tumoral excision |
| spellingShingle | Antonis Perdiou Ramona Dumitrescu Daniela Jumanca Octavia Balean Ruxandra Sava-Rosianu Serban Talpos Dacian Virgil Lalescu Atena Galuscan Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision Dentistry Journal autofluorescence oral cancer prevention margins of tumoral excision |
| title | Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision |
| title_full | Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision |
| title_fullStr | Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision |
| title_full_unstemmed | Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision |
| title_short | Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision |
| title_sort | leveraging autofluorescence for tumor detection diagnosis and accurate excision with surgical margin assessment in tumor excision |
| topic | autofluorescence oral cancer prevention margins of tumoral excision |
| url | https://www.mdpi.com/2304-6767/13/1/10 |
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