Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations
Chronic and acute nonhealing wounds represent a major public health problem, and replacement of cutaneous lesions by the newly regenerated skin is challenging. Mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) were separately tested in the attempt to regenerate the lost skin. However, thes...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/1234263 |
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| author | Barbara Hersant Mounia Sid-Ahmed Laura Braud Maud Jourdan Yasmine Baba-Amer Jean-Paul Meningaud Anne-Marie Rodriguez |
| author_facet | Barbara Hersant Mounia Sid-Ahmed Laura Braud Maud Jourdan Yasmine Baba-Amer Jean-Paul Meningaud Anne-Marie Rodriguez |
| author_sort | Barbara Hersant |
| collection | DOAJ |
| description | Chronic and acute nonhealing wounds represent a major public health problem, and replacement of cutaneous lesions by the newly regenerated skin is challenging. Mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) were separately tested in the attempt to regenerate the lost skin. However, these treatments often remained inefficient to achieve complete wound healing. Additional studies suggested that PRP could be used in combination with MSC to improve the cell therapy efficacy for tissue repair. However, systematic studies related to the effects of PRP on MSC properties and their ability to rebuild skin barrier are lacking. We evaluated in a mouse exhibiting 4 full-thickness wounds, the skin repair ability of a treatment combining human adipose-derived MSC and human PRP by comparison to treatment with saline solution, PRP alone, or MSC alone. Wound healing in these animals was measured at day 3, day 7, and day 10. In addition, we examined in vitro and in vivo whether PRP alters in MSC their proangiogenic properties, their survival, and their proliferation. We showed that PRP improved the efficacy of engrafted MSC to replace lost skin in mice by accelerating the wound healing processes and ameliorating the elasticity of the newly regenerated skin. In addition, we found that PRP treatment stimulated in vitro, in a dose-dependent manner, the proangiogenic potential of MSC through enhanced secretion of soluble factors like VEGF and SDF-1. Moreover, PRP treatment ameliorated the survival and activated the proliferation of in vitro cultured MSC and that these effects were accompanied by an alteration of the MSC energetic metabolism including oxygen consumption rate and mitochondrial ATP production. Similar observations were found in vivo following combined administration of PRP and MSC into mouse wounds. In conclusion, our study strengthens that the use of PRP in combination with MSC might be a safe alternative to aid wound healing. |
| format | Article |
| id | doaj-art-8f48d45a24d84b4b9dc7b8fb46bbe45f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8f48d45a24d84b4b9dc7b8fb46bbe45f2025-02-03T05:55:18ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/12342631234263Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism AlterationsBarbara Hersant0Mounia Sid-Ahmed1Laura Braud2Maud Jourdan3Yasmine Baba-Amer4Jean-Paul Meningaud5Anne-Marie Rodriguez6Université Paris-Est, UMR-S955, UPEC, Créteil, FranceAP-HP, Hôpital Henri Mondor-A. Chenevier, Service de Chirurgie Plastique et Maxillo-Faciale, Créteil, FranceUniversité Paris-Est, UMR-S955, UPEC, Créteil, FranceUniversité Paris-Est, UMR-S955, UPEC, Créteil, FranceUniversité Paris-Est, UMR-S955, UPEC, Créteil, FranceAP-HP, Hôpital Henri Mondor-A. Chenevier, Service de Chirurgie Plastique et Maxillo-Faciale, Créteil, FranceUniversité Paris-Est, UMR-S955, UPEC, Créteil, FranceChronic and acute nonhealing wounds represent a major public health problem, and replacement of cutaneous lesions by the newly regenerated skin is challenging. Mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) were separately tested in the attempt to regenerate the lost skin. However, these treatments often remained inefficient to achieve complete wound healing. Additional studies suggested that PRP could be used in combination with MSC to improve the cell therapy efficacy for tissue repair. However, systematic studies related to the effects of PRP on MSC properties and their ability to rebuild skin barrier are lacking. We evaluated in a mouse exhibiting 4 full-thickness wounds, the skin repair ability of a treatment combining human adipose-derived MSC and human PRP by comparison to treatment with saline solution, PRP alone, or MSC alone. Wound healing in these animals was measured at day 3, day 7, and day 10. In addition, we examined in vitro and in vivo whether PRP alters in MSC their proangiogenic properties, their survival, and their proliferation. We showed that PRP improved the efficacy of engrafted MSC to replace lost skin in mice by accelerating the wound healing processes and ameliorating the elasticity of the newly regenerated skin. In addition, we found that PRP treatment stimulated in vitro, in a dose-dependent manner, the proangiogenic potential of MSC through enhanced secretion of soluble factors like VEGF and SDF-1. Moreover, PRP treatment ameliorated the survival and activated the proliferation of in vitro cultured MSC and that these effects were accompanied by an alteration of the MSC energetic metabolism including oxygen consumption rate and mitochondrial ATP production. Similar observations were found in vivo following combined administration of PRP and MSC into mouse wounds. In conclusion, our study strengthens that the use of PRP in combination with MSC might be a safe alternative to aid wound healing.http://dx.doi.org/10.1155/2019/1234263 |
| spellingShingle | Barbara Hersant Mounia Sid-Ahmed Laura Braud Maud Jourdan Yasmine Baba-Amer Jean-Paul Meningaud Anne-Marie Rodriguez Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations Stem Cells International |
| title | Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations |
| title_full | Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations |
| title_fullStr | Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations |
| title_full_unstemmed | Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations |
| title_short | Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations |
| title_sort | platelet rich plasma improves the wound healing potential of mesenchymal stem cells through paracrine and metabolism alterations |
| url | http://dx.doi.org/10.1155/2019/1234263 |
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