Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans
Introduction. The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2019/5398732 |
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| author | Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Max Efui Annani-Akollor Kwabena Owusu Danquah Lillian Boateng Eddie-Williams Owiredu Edward Y. Afriyie Richard Vikpebah Duneeh Renate Asare David Ofosu Ntiamoah Richard Boateng |
| author_facet | Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Max Efui Annani-Akollor Kwabena Owusu Danquah Lillian Boateng Eddie-Williams Owiredu Edward Y. Afriyie Richard Vikpebah Duneeh Renate Asare David Ofosu Ntiamoah Richard Boateng |
| author_sort | Otchere Addai-Mensah |
| collection | DOAJ |
| description | Introduction. The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans. Methods. A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method. Results. Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032). Conclusion. Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia. |
| format | Article |
| id | doaj-art-8ef760e5ac734036b6c4fd8192045f3f |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8ef760e5ac734036b6c4fd8192045f3f2025-02-03T01:26:15ZengWileyThe Scientific World Journal2356-61401537-744X2019-01-01201910.1155/2019/53987325398732Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in HumansOtchere Addai-Mensah0Daniel Gyamfi1Francis Agyei Amponsah2Max Efui Annani-Akollor3Kwabena Owusu Danquah4Lillian Boateng5Eddie-Williams Owiredu6Edward Y. Afriyie7Richard Vikpebah Duneeh8Renate Asare9David Ofosu Ntiamoah10Richard Boateng11Department of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaSt. John of God Hospital, Duayaw Nkwanta, Sunyani, GhanaDepartment of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Medical Laboratory Technology, Faculty of Allied Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Haematology, Komfo Anokye Teaching Hospital, Kumasi, GhanaDepartment of Haematology, Komfo Anokye Teaching Hospital, Kumasi, GhanaDepartment of Haematology, Komfo Anokye Teaching Hospital, Kumasi, GhanaDepartment of Haematology, Komfo Anokye Teaching Hospital, Kumasi, GhanaDepartment of Serology, Komfo Anokye Teaching Hospital, Kumasi, GhanaIntroduction. The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans. Methods. A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method. Results. Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032). Conclusion. Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia.http://dx.doi.org/10.1155/2019/5398732 |
| spellingShingle | Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Max Efui Annani-Akollor Kwabena Owusu Danquah Lillian Boateng Eddie-Williams Owiredu Edward Y. Afriyie Richard Vikpebah Duneeh Renate Asare David Ofosu Ntiamoah Richard Boateng Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans The Scientific World Journal |
| title | Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans |
| title_full | Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans |
| title_fullStr | Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans |
| title_full_unstemmed | Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans |
| title_short | Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans |
| title_sort | antierythropoietin antibody production is not associated with malaria and malaria related anaemia in humans |
| url | http://dx.doi.org/10.1155/2019/5398732 |
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