Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates

Genetic polymorphisms in the gene that codes for endothelial nitric oxide synthase (eNOS) have been associated with less nitric oxide availability and with various cardiovascular diseases in humans. The objective of this study was to analyze the genotype distributions and allele frequencies f...

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Main Authors: Ercan Sivasli, Melih Babaoğlu, Umit Yaşar, Murat Yurdakök, Atila Bozkurt, Ayşe Korkmaz, Sule Yiğit, Gülsevin Tekinalp
Format: Article
Language:English
Published: Hacettepe University Institute of Child Health 2010-04-01
Series:The Turkish Journal of Pediatrics
Online Access:https://turkjpediatr.org/article/view/4977
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author Ercan Sivasli
Melih Babaoğlu
Umit Yaşar
Murat Yurdakök
Atila Bozkurt
Ayşe Korkmaz
Sule Yiğit
Gülsevin Tekinalp
author_facet Ercan Sivasli
Melih Babaoğlu
Umit Yaşar
Murat Yurdakök
Atila Bozkurt
Ayşe Korkmaz
Sule Yiğit
Gülsevin Tekinalp
author_sort Ercan Sivasli
collection DOAJ
description Genetic polymorphisms in the gene that codes for endothelial nitric oxide synthase (eNOS) have been associated with less nitric oxide availability and with various cardiovascular diseases in humans. The objective of this study was to analyze the genotype distributions and allele frequencies for the Glu298Asp (G894T) and T(-786)C polymorphisms of the eNOS gene among neonates with respiratory distress in comparison to healthy control subjects. Fifty premature neonates with respiratory distress and 55 neonates without any respiratory problem were included in the study. Genomic DNA from all the neonates was analyzed by polymerase chain reaction. A polymerase chain reaction-restriction fragment length polymorphism analysis of eNOS gene polymorphisms was performed, and the results were compared. There were no significant differences between the groups regarding either genotype distributions or the allele frequencies for the Glu298Asp and T(-786)C polymorphisms. These results suggest that eNOS Glu298Asp and T(-786)C polymorphisms are not associated with development of respiratory distress.
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language English
publishDate 2010-04-01
publisher Hacettepe University Institute of Child Health
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series The Turkish Journal of Pediatrics
spelling doaj-art-8ed7f4be11a24b0a83c76d9946aae7b82025-08-20T02:01:53ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212010-04-01522Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonatesErcan Sivasli0Melih BabaoğluUmit YaşarMurat YurdakökAtila BozkurtAyşe KorkmazSule YiğitGülsevin TekinalpDepartment of Pediatrics, Hacettepe University Faculty of Medicine, Ankara, Turkey. Genetic polymorphisms in the gene that codes for endothelial nitric oxide synthase (eNOS) have been associated with less nitric oxide availability and with various cardiovascular diseases in humans. The objective of this study was to analyze the genotype distributions and allele frequencies for the Glu298Asp (G894T) and T(-786)C polymorphisms of the eNOS gene among neonates with respiratory distress in comparison to healthy control subjects. Fifty premature neonates with respiratory distress and 55 neonates without any respiratory problem were included in the study. Genomic DNA from all the neonates was analyzed by polymerase chain reaction. A polymerase chain reaction-restriction fragment length polymorphism analysis of eNOS gene polymorphisms was performed, and the results were compared. There were no significant differences between the groups regarding either genotype distributions or the allele frequencies for the Glu298Asp and T(-786)C polymorphisms. These results suggest that eNOS Glu298Asp and T(-786)C polymorphisms are not associated with development of respiratory distress. https://turkjpediatr.org/article/view/4977
spellingShingle Ercan Sivasli
Melih Babaoğlu
Umit Yaşar
Murat Yurdakök
Atila Bozkurt
Ayşe Korkmaz
Sule Yiğit
Gülsevin Tekinalp
Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
The Turkish Journal of Pediatrics
title Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
title_full Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
title_fullStr Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
title_full_unstemmed Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
title_short Association between the Glu298Asp and T(-786)C polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
title_sort association between the glu298asp and t 786 c polymorphisms of the endothelial nitric oxide synthase gene and respiratory distress in preterm neonates
url https://turkjpediatr.org/article/view/4977
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