The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model
Introduction : Pneumoperitoneum (PP) is known to cause ischemia in kidneys and other intra-abdominal organs because of decreased splanchnic blood flow. Aim : We aimed to determine the degree of renal injury that occurs due to a PP and prolonged PP. We measured renal injury biomarkers and made a h...
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Medycyna Praktyczna
2017-04-01
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| Series: | Videosurgery and Other Miniinvasive Techniques |
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| Online Access: | https://www.termedia.pl/The-effects-of-prolonged-CO-2-insufflation-on-kidney-function-in-a-rat-pneumoperitoneum-model,42,29817,1,1.html |
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| author | Dogan Yildirim Turgut Donmez Oguzhan Sunamak Semih Mirapoglu Adnan Hut Nilgun Rukiye Erdogan Zumrut Mine Isık Saglam Huseyin Kilincaslan |
| author_facet | Dogan Yildirim Turgut Donmez Oguzhan Sunamak Semih Mirapoglu Adnan Hut Nilgun Rukiye Erdogan Zumrut Mine Isık Saglam Huseyin Kilincaslan |
| author_sort | Dogan Yildirim |
| collection | DOAJ |
| description | Introduction : Pneumoperitoneum (PP) is known to cause ischemia in kidneys and other intra-abdominal organs because of decreased splanchnic blood flow.
Aim : We aimed to determine the degree of renal injury that occurs due to a PP and prolonged PP. We measured renal injury biomarkers and made a histopathological evaluation to estimate the degree of injury and assessed the correlation of biomarkers with histopathological findings.
Material and methods : Twenty-one female Sprague Dawley rats were separated randomly into three groups. Group 1 was the control group and was given anesthesia for 3 h. In group 2, a PP was administered under anesthesia for 1 h. A pneumoperitoneum was administered under anesthesia to animals in group 3 for 3 h.
Results : Pathological analysis showed a significant statistical difference between the 3 groups. In particular, neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (Cys C) levels at the 24th h and preoperative mean urea levels showed a significant difference between the groups. The 24th-hour NGAL level in group 3 was significantly higher than that of group 1. The preoperative Cys C level was higher in group 1 than in either group 2 or 3. Cys C was decreased significantly in group 1 and increased significantly in both groups 2 and 3.
Conclusions : The increase in NGAL and Cys C levels directly correlated with the duration of PP and intra-abdominal pressure, and they are therefore good biomarkers in diagnosing acute renal injury in the early phase. Serum creatinine level is not a good biomarker in the early phase of renal injury. |
| format | Article |
| id | doaj-art-8e9de9ca48024fb68bec6d9498ce3b4a |
| institution | DOAJ |
| issn | 1895-4588 2299-0054 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | Medycyna Praktyczna |
| record_format | Article |
| series | Videosurgery and Other Miniinvasive Techniques |
| spelling | doaj-art-8e9de9ca48024fb68bec6d9498ce3b4a2025-08-20T03:07:17ZengMedycyna PraktycznaVideosurgery and Other Miniinvasive Techniques1895-45882299-00542017-04-0112212513410.5114/wiitm.2017.6721029817The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum modelDogan YildirimTurgut DonmezOguzhan SunamakSemih MirapogluAdnan HutNilgun Rukiye ErdoganZumrut Mine Isık SaglamHuseyin KilincaslanIntroduction : Pneumoperitoneum (PP) is known to cause ischemia in kidneys and other intra-abdominal organs because of decreased splanchnic blood flow. Aim : We aimed to determine the degree of renal injury that occurs due to a PP and prolonged PP. We measured renal injury biomarkers and made a histopathological evaluation to estimate the degree of injury and assessed the correlation of biomarkers with histopathological findings. Material and methods : Twenty-one female Sprague Dawley rats were separated randomly into three groups. Group 1 was the control group and was given anesthesia for 3 h. In group 2, a PP was administered under anesthesia for 1 h. A pneumoperitoneum was administered under anesthesia to animals in group 3 for 3 h. Results : Pathological analysis showed a significant statistical difference between the 3 groups. In particular, neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (Cys C) levels at the 24th h and preoperative mean urea levels showed a significant difference between the groups. The 24th-hour NGAL level in group 3 was significantly higher than that of group 1. The preoperative Cys C level was higher in group 1 than in either group 2 or 3. Cys C was decreased significantly in group 1 and increased significantly in both groups 2 and 3. Conclusions : The increase in NGAL and Cys C levels directly correlated with the duration of PP and intra-abdominal pressure, and they are therefore good biomarkers in diagnosing acute renal injury in the early phase. Serum creatinine level is not a good biomarker in the early phase of renal injury.https://www.termedia.pl/The-effects-of-prolonged-CO-2-insufflation-on-kidney-function-in-a-rat-pneumoperitoneum-model,42,29817,1,1.html<i>pneumoperitoneum neutrophil gelatinase-associated lipocalin cystatin C acute kidney injury rats</i> |
| spellingShingle | Dogan Yildirim Turgut Donmez Oguzhan Sunamak Semih Mirapoglu Adnan Hut Nilgun Rukiye Erdogan Zumrut Mine Isık Saglam Huseyin Kilincaslan The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model Videosurgery and Other Miniinvasive Techniques <i>pneumoperitoneum neutrophil gelatinase-associated lipocalin cystatin C acute kidney injury rats</i> |
| title | The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model |
| title_full | The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model |
| title_fullStr | The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model |
| title_full_unstemmed | The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model |
| title_short | The effects of prolonged CO 2 insufflation on kidney function in a rat pneumoperitoneum model |
| title_sort | effects of prolonged co 2 insufflation on kidney function in a rat pneumoperitoneum model |
| topic | <i>pneumoperitoneum neutrophil gelatinase-associated lipocalin cystatin C acute kidney injury rats</i> |
| url | https://www.termedia.pl/The-effects-of-prolonged-CO-2-insufflation-on-kidney-function-in-a-rat-pneumoperitoneum-model,42,29817,1,1.html |
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