Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus

Motile Aeromonad Septicaemia (MAS) disease is one of bacterial disease frequently infecting freshwater fishes including patin catfish Pangasianodon hypophthalmus.  This study was performed to determine antimicrobial of Phaleria macrocarpa (PM) and its potency against MAS disease caused by Aeromonas...

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Main Authors: D. Wahjuningrum, S.L. Angka, W. Lesmanawati, . Sa’diyah, M. Yuhana
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2007-01-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/4017
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author D. Wahjuningrum
S.L. Angka
W. Lesmanawati
. Sa’diyah
M. Yuhana
author_facet D. Wahjuningrum
S.L. Angka
W. Lesmanawati
. Sa’diyah
M. Yuhana
author_sort D. Wahjuningrum
collection DOAJ
description Motile Aeromonad Septicaemia (MAS) disease is one of bacterial disease frequently infecting freshwater fishes including patin catfish Pangasianodon hypophthalmus.  This study was performed to determine antimicrobial of Phaleria macrocarpa (PM) and its potency against MAS disease caused by Aeromonas hydrophila.  The in vitro susceptibility test was performed by pour plate methods at the dosages of 2, 4, 6, 8, and 10 g/l PM. At the in vivo test, fish were fed with the addition of PM into the diet at a dosage of 6, 12, and 18 g/l and 0 g/l as a control for 8 days. At ninth day, fish were infected with A.hydrophila. For seven days after infection the clinical signs and blood pictures were observed. The in vitro test indicated that PM had an antibacterial effect to A.hydrophila at the dosage of 6 g/l. Addition of PM in the diet for 8 days increased haemoglobine. The results showed that lowest clinical sign and smallest number of in fected fish was found at dosage of 12 g/l PM. PM can be used as a preventive method for MAS. Keywords:  Phaleria macrocarpa, antibacterial, "patin", MAS disease, Aeromonas hydrophila   Abstrak Penyakit MAS (Motile Aeromonad Septicaemia) merupakan penyakit bakterial yang banyak menyerang ikan-ikan air tawar termasuk patin Pangasianodon hypophthalmus. Penelitian ini bertujuan untuk melihat kemampuan antibakteri dari mahkota dewa (MD) Phaleria macrocarpa terhadap Aeromonas hydrophila penyebab penyakit MAS dan potensinya dalam pencegahan penyakit ini.  Pada uji in vitro dilakukan pengujian aktivitas antibakteri MD terhadap A. hydrophila dengan metode hitungan cawan pada dosis MD 2, 4, 6, 8, dan 10 g/l. Pada uji in vivo, ikan uji diberi pakan yang dicampur MD dengan dosis berbeda yaitu 0 g/l (kontrol +), 6, 12, dan 18 g/l, selama 8 hari. Pada hari kesembilan ikan disuntik dengan A. hydrophila dan pengamatan dilanjutkan selama 7 hari, meliputi pengamatan gejala klinis dan gambaran darah.  Hasil penelitian menunjukkan bahwa MD bersifat antibakteri terhadap A. hydrophila dengan dosis efektif 6 g/l. Pemberian MD selama 8 hari dapat meningkatkan kadar hemoglobin, kadar hematokrit, jumlah lekosit, serta meningkatkan kemampuan fagositik darah. Dosis MD sebesar 12 g/l menunjukkan hasil paling baik yang ditunjang oleh gejala klinis paling ringan (sampai tahap nekrosis), dengan jumlah ikan yang terinfeksi paling sedikit (45%) dan waktu penyembuhan paling cepat (hari ke 6). Dengan demikian, MD dapat digunakan untuk mencegah penyakit MAS. Kata kunci:  mahkota dewa, antibakterial, ikan patin, penyakit MAS, Aeromonas hydrophila
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spelling doaj-art-8e869f0387a94d19bbb3a7eed62cbc9e2025-01-24T18:05:02ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-01-016110.19027/jai.6.109-1173626Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmusD. Wahjuningrum0S.L. Angka1W. Lesmanawati2. Sa’diyah3M. Yuhana4Bogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of Aquaculture Motile Aeromonad Septicaemia (MAS) disease is one of bacterial disease frequently infecting freshwater fishes including patin catfish Pangasianodon hypophthalmus.  This study was performed to determine antimicrobial of Phaleria macrocarpa (PM) and its potency against MAS disease caused by Aeromonas hydrophila.  The in vitro susceptibility test was performed by pour plate methods at the dosages of 2, 4, 6, 8, and 10 g/l PM. At the in vivo test, fish were fed with the addition of PM into the diet at a dosage of 6, 12, and 18 g/l and 0 g/l as a control for 8 days. At ninth day, fish were infected with A.hydrophila. For seven days after infection the clinical signs and blood pictures were observed. The in vitro test indicated that PM had an antibacterial effect to A.hydrophila at the dosage of 6 g/l. Addition of PM in the diet for 8 days increased haemoglobine. The results showed that lowest clinical sign and smallest number of in fected fish was found at dosage of 12 g/l PM. PM can be used as a preventive method for MAS. Keywords:  Phaleria macrocarpa, antibacterial, "patin", MAS disease, Aeromonas hydrophila   Abstrak Penyakit MAS (Motile Aeromonad Septicaemia) merupakan penyakit bakterial yang banyak menyerang ikan-ikan air tawar termasuk patin Pangasianodon hypophthalmus. Penelitian ini bertujuan untuk melihat kemampuan antibakteri dari mahkota dewa (MD) Phaleria macrocarpa terhadap Aeromonas hydrophila penyebab penyakit MAS dan potensinya dalam pencegahan penyakit ini.  Pada uji in vitro dilakukan pengujian aktivitas antibakteri MD terhadap A. hydrophila dengan metode hitungan cawan pada dosis MD 2, 4, 6, 8, dan 10 g/l. Pada uji in vivo, ikan uji diberi pakan yang dicampur MD dengan dosis berbeda yaitu 0 g/l (kontrol +), 6, 12, dan 18 g/l, selama 8 hari. Pada hari kesembilan ikan disuntik dengan A. hydrophila dan pengamatan dilanjutkan selama 7 hari, meliputi pengamatan gejala klinis dan gambaran darah.  Hasil penelitian menunjukkan bahwa MD bersifat antibakteri terhadap A. hydrophila dengan dosis efektif 6 g/l. Pemberian MD selama 8 hari dapat meningkatkan kadar hemoglobin, kadar hematokrit, jumlah lekosit, serta meningkatkan kemampuan fagositik darah. Dosis MD sebesar 12 g/l menunjukkan hasil paling baik yang ditunjang oleh gejala klinis paling ringan (sampai tahap nekrosis), dengan jumlah ikan yang terinfeksi paling sedikit (45%) dan waktu penyembuhan paling cepat (hari ke 6). Dengan demikian, MD dapat digunakan untuk mencegah penyakit MAS. Kata kunci:  mahkota dewa, antibakterial, ikan patin, penyakit MAS, Aeromonas hydrophila https://journal.ipb.ac.id/index.php/jai/article/view/4017
spellingShingle D. Wahjuningrum
S.L. Angka
W. Lesmanawati
. Sa’diyah
M. Yuhana
Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
Jurnal Akuakultur Indonesia
title Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
title_full Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
title_fullStr Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
title_full_unstemmed Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
title_short Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
title_sort prospect use of phaleria macrocarpa to prevent motile aeromonad septicaemia disease in patin catfish pangasianodon hypophthalmus
url https://journal.ipb.ac.id/index.php/jai/article/view/4017
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