Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations
Abstract Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s40104-024-01144-y |
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| author | Ezequias Castillo-Lopez Patrick Biber Arife Sener-Aydemir Karin Hummel Ebrahim Razzazi-Fazeli Nicole Reisinger Qendrim Zebeli Susanne Kreuzer-Redmer Thomas Hartinger |
| author_facet | Ezequias Castillo-Lopez Patrick Biber Arife Sener-Aydemir Karin Hummel Ebrahim Razzazi-Fazeli Nicole Reisinger Qendrim Zebeli Susanne Kreuzer-Redmer Thomas Hartinger |
| author_sort | Ezequias Castillo-Lopez |
| collection | DOAJ |
| description | Abstract Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified through cluster analysis and were identified as poor, average, and excellent quality. The proteome was assessed with quantitative shotgun proteomics; abundance data were compared among the colostrum types; enrichment analysis of metabolic processes and proteins classes was performed as well. We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves. Results On average, 428 proteins were identified per sample, which belonged mainly to cellular process, biological regulation, response to stimulus, metabolic process, and immune system process. Most abundant proteins were complement C3 (Q2UVX4), alpha-S1-casein (P02662), Ig-like domain-containing protein (A0A3Q1M032), albumin (A0A140T897), polymeric immunoglobulin receptor (P81265), lactotransferrrin (P24627), and IGHG1*01 (X16701_4). Colostrum of excellent quality had greater (P < 0.05) abundance of serpin A3-7 (A2I7N3), complement factor I (A0A3Q1MIF4), lipocalin/cytosolic fatty-acid binding domain-containing protein (A0A3Q1MRQ2), complement C3 (E1B805), complement component 4 binding protein alpha (A0AAF6ZHP5), and complement component C6 (F1MM86). However, colostrum of excellent quality had lower (P < 0.05) abundance of HGF activator (E1BCW0), alpha-S1-casein (P02662), and xanthine dehydrogenase/oxidase (P80457). This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway, complement activation, complement activation classical pathway, humoral immune response, leukocyte mediated immunity, and negative regulation of endopeptidase activity in excellent-quality colostrum. Additionally, some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of calves (P < 0.05; r ≥ 0.57). Conclusions This study provides new insights into the bovine colostrum proteome, demonstrating associations between IgG levels and the abundance of other proteins, as well as the enrichment of metabolic processes related to innate immune response. Thus, results suggest that the colostrum proteomic profile is associated with the content of IgG. Future research should deeply explore the association of these findings with pre-calving nutrition status and blood composition of the cow, and with passive immunity transfer to the calf. |
| format | Article |
| id | doaj-art-8e717a51742741ea83330e9110cb3707 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-8e717a51742741ea83330e9110cb37072025-01-26T12:46:32ZengBMCJournal of Animal Science and Biotechnology2049-18912025-01-0116111710.1186/s40104-024-01144-yCharacterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrationsEzequias Castillo-Lopez0Patrick Biber1Arife Sener-Aydemir2Karin Hummel3Ebrahim Razzazi-Fazeli4Nicole Reisinger5Qendrim Zebeli6Susanne Kreuzer-Redmer7Thomas Hartinger8Center for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaCenter for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaCenter for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaUniversity of Veterinary Medicine Vienna, VetCore Facility (Mass Spectrometry)University of Veterinary Medicine Vienna, VetCore Facility (Mass Spectrometry)Dsm-Firmenich, Animal Nutrition & Health R&D CenterCenter for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaCenter for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaCenter for Animal Nutrition and Welfare, University of Veterinary Medicine ViennaAbstract Background The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified through cluster analysis and were identified as poor, average, and excellent quality. The proteome was assessed with quantitative shotgun proteomics; abundance data were compared among the colostrum types; enrichment analysis of metabolic processes and proteins classes was performed as well. We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves. Results On average, 428 proteins were identified per sample, which belonged mainly to cellular process, biological regulation, response to stimulus, metabolic process, and immune system process. Most abundant proteins were complement C3 (Q2UVX4), alpha-S1-casein (P02662), Ig-like domain-containing protein (A0A3Q1M032), albumin (A0A140T897), polymeric immunoglobulin receptor (P81265), lactotransferrrin (P24627), and IGHG1*01 (X16701_4). Colostrum of excellent quality had greater (P < 0.05) abundance of serpin A3-7 (A2I7N3), complement factor I (A0A3Q1MIF4), lipocalin/cytosolic fatty-acid binding domain-containing protein (A0A3Q1MRQ2), complement C3 (E1B805), complement component 4 binding protein alpha (A0AAF6ZHP5), and complement component C6 (F1MM86). However, colostrum of excellent quality had lower (P < 0.05) abundance of HGF activator (E1BCW0), alpha-S1-casein (P02662), and xanthine dehydrogenase/oxidase (P80457). This resulted in enrichment of the biological processes predominantly for complement activation alternative pathway, complement activation, complement activation classical pathway, humoral immune response, leukocyte mediated immunity, and negative regulation of endopeptidase activity in excellent-quality colostrum. Additionally, some colostrum proteins were found to be correlated with the blood globulin level of cows and with the passive immunity level of calves (P < 0.05; r ≥ 0.57). Conclusions This study provides new insights into the bovine colostrum proteome, demonstrating associations between IgG levels and the abundance of other proteins, as well as the enrichment of metabolic processes related to innate immune response. Thus, results suggest that the colostrum proteomic profile is associated with the content of IgG. Future research should deeply explore the association of these findings with pre-calving nutrition status and blood composition of the cow, and with passive immunity transfer to the calf.https://doi.org/10.1186/s40104-024-01144-yBovine colostrumCowImmunoglobulinsPassive immunityProteomics |
| spellingShingle | Ezequias Castillo-Lopez Patrick Biber Arife Sener-Aydemir Karin Hummel Ebrahim Razzazi-Fazeli Nicole Reisinger Qendrim Zebeli Susanne Kreuzer-Redmer Thomas Hartinger Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations Journal of Animal Science and Biotechnology Bovine colostrum Cow Immunoglobulins Passive immunity Proteomics |
| title | Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations |
| title_full | Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations |
| title_fullStr | Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations |
| title_full_unstemmed | Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations |
| title_short | Characterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations |
| title_sort | characterization of the colostrum proteome of primiparous holstein cows and its association with colostrum immunoglobulin g concentrations |
| topic | Bovine colostrum Cow Immunoglobulins Passive immunity Proteomics |
| url | https://doi.org/10.1186/s40104-024-01144-y |
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