Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit
Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolatio...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2015/280784 |
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| author | B. E. Rangaswamy K. P. Vanitha Basavaraj S. Hungund |
| author_facet | B. E. Rangaswamy K. P. Vanitha Basavaraj S. Hungund |
| author_sort | B. E. Rangaswamy |
| collection | DOAJ |
| description | Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified as Gluconacetobacter sp. RV28, Enterobacter sp. RV11, and Pseudomonas sp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources using Gluconacetobacter sp. RV28. The strain produced 4.7 g/L of cellulose at optimum growth conditions of temperature (30°C), pH (6.0), sucrose (2%), peptone (0.5%), and inoculum density (5%). Characterization of microbial cellulose was done by scanning electron microscopy (SEM). |
| format | Article |
| id | doaj-art-8e70912b855246e6b512d452f02dc872 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-8e70912b855246e6b512d452f02dc8722025-02-03T01:02:24ZengWileyInternational Journal of Polymer Science1687-94221687-94302015-01-01201510.1155/2015/280784280784Microbial Cellulose Production from Bacteria Isolated from Rotten FruitB. E. Rangaswamy0K. P. Vanitha1Basavaraj S. Hungund2Department of Biotechnology, Bapuji Institute of Engineering and Technology, Davangere 577004, IndiaDepartment of Biotechnology, Bapuji Institute of Engineering and Technology, Davangere 577004, IndiaDepartment of Biotechnology, B.V.B. College of Engineering and Technology, Hubballi 580031, IndiaMicrobial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified as Gluconacetobacter sp. RV28, Enterobacter sp. RV11, and Pseudomonas sp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources using Gluconacetobacter sp. RV28. The strain produced 4.7 g/L of cellulose at optimum growth conditions of temperature (30°C), pH (6.0), sucrose (2%), peptone (0.5%), and inoculum density (5%). Characterization of microbial cellulose was done by scanning electron microscopy (SEM).http://dx.doi.org/10.1155/2015/280784 |
| spellingShingle | B. E. Rangaswamy K. P. Vanitha Basavaraj S. Hungund Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit International Journal of Polymer Science |
| title | Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit |
| title_full | Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit |
| title_fullStr | Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit |
| title_full_unstemmed | Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit |
| title_short | Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit |
| title_sort | microbial cellulose production from bacteria isolated from rotten fruit |
| url | http://dx.doi.org/10.1155/2015/280784 |
| work_keys_str_mv | AT berangaswamy microbialcelluloseproductionfrombacteriaisolatedfromrottenfruit AT kpvanitha microbialcelluloseproductionfrombacteriaisolatedfromrottenfruit AT basavarajshungund microbialcelluloseproductionfrombacteriaisolatedfromrottenfruit |