Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites
Recent studies propose the existence of a blood microbiome, but its composition, origin, and dynamics remain largely unresolved. In this pilot study, we analyzed the bacterial DNA present in the blood of 10 volunteers by comparing the taxonomic profiles of 16S rRNA gene sequences from skin, vaginal,...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes Reports |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/29933935.2025.2482771 |
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| author | Maija Rozenberga Rihards Saksis Ilze Elbere Liga Birzniece Monta Briviba Ilze Konrade Janis Klovins |
| author_facet | Maija Rozenberga Rihards Saksis Ilze Elbere Liga Birzniece Monta Briviba Ilze Konrade Janis Klovins |
| author_sort | Maija Rozenberga |
| collection | DOAJ |
| description | Recent studies propose the existence of a blood microbiome, but its composition, origin, and dynamics remain largely unresolved. In this pilot study, we analyzed the bacterial DNA present in the blood of 10 volunteers by comparing the taxonomic profiles of 16S rRNA gene sequences from skin, vaginal, oral, and fecal samples. After applying stringent decontamination protocols, we detected bacterial DNA in all blood samples, predominantly from the Pseudomonas genus. A key finding was the identification of 32 unique Amplicon Sequence Variants (ASVs) that were identical between blood and a single body site within individual participants, with no overlap between multiple body sites or across different participants. This participant-specific overlap suggests a true biological origin of bacterial DNA in blood, likely stemming from localized bacterial migration, such as from the skin. Additionally, 27.4% of the ASVs in blood were found in other body sites, with the highest overlap observed in skin samples. Furthermore, 25.3% of blood ASVs persisted after three months, suggesting a consistent pattern in the bacterial DNA composition detected in blood over time. These findings deepen our understanding of the blood microbiome and provide a basis for future research linking blood microbiota to health and disease phenotypes. |
| format | Article |
| id | doaj-art-8e66cb481e0f4e53bc8173f9b8ab2b5f |
| institution | OA Journals |
| issn | 2993-3935 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes Reports |
| spelling | doaj-art-8e66cb481e0f4e53bc8173f9b8ab2b5f2025-08-20T02:12:41ZengTaylor & Francis GroupGut Microbes Reports2993-39352025-12-012110.1080/29933935.2025.2482771Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sitesMaija Rozenberga0Rihards Saksis1Ilze Elbere2Liga Birzniece3Monta Briviba4Ilze Konrade5Janis Klovins6Latvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaLatvian Biomedical Research and Study Centre, Translational Omics group, Riga, LatviaRecent studies propose the existence of a blood microbiome, but its composition, origin, and dynamics remain largely unresolved. In this pilot study, we analyzed the bacterial DNA present in the blood of 10 volunteers by comparing the taxonomic profiles of 16S rRNA gene sequences from skin, vaginal, oral, and fecal samples. After applying stringent decontamination protocols, we detected bacterial DNA in all blood samples, predominantly from the Pseudomonas genus. A key finding was the identification of 32 unique Amplicon Sequence Variants (ASVs) that were identical between blood and a single body site within individual participants, with no overlap between multiple body sites or across different participants. This participant-specific overlap suggests a true biological origin of bacterial DNA in blood, likely stemming from localized bacterial migration, such as from the skin. Additionally, 27.4% of the ASVs in blood were found in other body sites, with the highest overlap observed in skin samples. Furthermore, 25.3% of blood ASVs persisted after three months, suggesting a consistent pattern in the bacterial DNA composition detected in blood over time. These findings deepen our understanding of the blood microbiome and provide a basis for future research linking blood microbiota to health and disease phenotypes.https://www.tandfonline.com/doi/10.1080/29933935.2025.2482771Blood microbiomecirculating microbiomecirculating microbial DNAmicrobiome stabilitymicrobiome origin |
| spellingShingle | Maija Rozenberga Rihards Saksis Ilze Elbere Liga Birzniece Monta Briviba Ilze Konrade Janis Klovins Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites Gut Microbes Reports Blood microbiome circulating microbiome circulating microbial DNA microbiome stability microbiome origin |
| title | Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites |
| title_full | Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites |
| title_fullStr | Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites |
| title_full_unstemmed | Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites |
| title_short | Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites |
| title_sort | tracking the origin of bacterial dna in blood indication of localized and sporadic transfer from other body sites |
| topic | Blood microbiome circulating microbiome circulating microbial DNA microbiome stability microbiome origin |
| url | https://www.tandfonline.com/doi/10.1080/29933935.2025.2482771 |
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