Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i>
Plant <i>cis</i>-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the...
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2025-01-01
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| Series: | Horticulturae |
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| author | Xingbin Lv Hua Yang Yufang Hu Qi Liang Shuyun Tian Lang Yang Mingkun Huang Ling Zhang Yanqin Xu |
| author_facet | Xingbin Lv Hua Yang Yufang Hu Qi Liang Shuyun Tian Lang Yang Mingkun Huang Ling Zhang Yanqin Xu |
| author_sort | Xingbin Lv |
| collection | DOAJ |
| description | Plant <i>cis</i>-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the <i>35S</i> promoter, to avoid some negative effects caused by exogenous DNA sequences. In this study, we provided a comprehensive analysis of the published genomic data of <i>Andrographis paniculata</i> and identified six candidate <i>ApACRs</i>. Based on the transient assays, two candidate CRSs (<i>ApACR1</i> and <i>ApACR2</i>) demonstrated high transcriptional activities comparable to those of the <i>35S</i> promoter in several plant species, in contrast to others with lower activities, suggesting their further potential applications in plant biotechnology and genetic engineering. |
| format | Article |
| id | doaj-art-8e607ab85f3842c9991526e2df0f695a |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-8e607ab85f3842c9991526e2df0f695a2025-01-24T13:34:39ZengMDPI AGHorticulturae2311-75242025-01-011116310.3390/horticulturae11010063Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i>Xingbin Lv0Hua Yang1Yufang Hu2Qi Liang3Shuyun Tian4Lang Yang5Mingkun Huang6Ling Zhang7Yanqin Xu8School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, 1688 Meiling Avenue, Xinjian District, Nanchang 330004, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaJiangxi Provincial Key Laboratory of Ex Situ Plant Conservation and Utilization, Lushan Botanical Garden, Chinese Academy of Sciences, 9 Zhiqing Road, Jiujiang 332900, ChinaSchool of Pharmacy, Jiangxi University of Traditional Chinese Medicine, 1688 Meiling Avenue, Xinjian District, Nanchang 330004, ChinaPlant <i>cis</i>-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the <i>35S</i> promoter, to avoid some negative effects caused by exogenous DNA sequences. In this study, we provided a comprehensive analysis of the published genomic data of <i>Andrographis paniculata</i> and identified six candidate <i>ApACRs</i>. Based on the transient assays, two candidate CRSs (<i>ApACR1</i> and <i>ApACR2</i>) demonstrated high transcriptional activities comparable to those of the <i>35S</i> promoter in several plant species, in contrast to others with lower activities, suggesting their further potential applications in plant biotechnology and genetic engineering.https://www.mdpi.com/2311-7524/11/1/63<i>Andrographis paniculata</i>medicinal plant<i>cis</i>-regulatory sequencesepigenic modificationstranscriptional regulation |
| spellingShingle | Xingbin Lv Hua Yang Yufang Hu Qi Liang Shuyun Tian Lang Yang Mingkun Huang Ling Zhang Yanqin Xu Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> Horticulturae <i>Andrographis paniculata</i> medicinal plant <i>cis</i>-regulatory sequences epigenic modifications transcriptional regulation |
| title | Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> |
| title_full | Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> |
| title_fullStr | Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> |
| title_full_unstemmed | Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> |
| title_short | Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> |
| title_sort | genomic characterization and functional validation of six i cis i regulatory sequences in medicinal plant i andrographis paniculata i |
| topic | <i>Andrographis paniculata</i> medicinal plant <i>cis</i>-regulatory sequences epigenic modifications transcriptional regulation |
| url | https://www.mdpi.com/2311-7524/11/1/63 |
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