Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i>

Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i>

Plant <i>cis</i>-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the...

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Main Authors: Xingbin Lv, Hua Yang, Yufang Hu, Qi Liang, Shuyun Tian, Lang Yang, Mingkun Huang, Ling Zhang, Yanqin Xu
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Horticulturae
Subjects:
<i>Andrographis paniculata</i>
medicinal plant
<i>cis</i>-regulatory sequences
epigenic modifications
transcriptional regulation
Online Access:https://www.mdpi.com/2311-7524/11/1/63
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Summary:Plant <i>cis</i>-regulatory sequences (CRSs) are essential for gene expression and transcriptional regulation in plants. With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the <i>35S</i> promoter, to avoid some negative effects caused by exogenous DNA sequences. In this study, we provided a comprehensive analysis of the published genomic data of <i>Andrographis paniculata</i> and identified six candidate <i>ApACRs</i>. Based on the transient assays, two candidate CRSs (<i>ApACR1</i> and <i>ApACR2</i>) demonstrated high transcriptional activities comparable to those of the <i>35S</i> promoter in several plant species, in contrast to others with lower activities, suggesting their further potential applications in plant biotechnology and genetic engineering.
ISSN:2311-7524

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