Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort
Rheumatoid arthritis (RA) is one of the complex diseases with the involvement of the genetic as well as environmental factors in its onset and severity. Different genome-wide association and candidate gene studies have shown the role of several genetic variants in multiple loci/genes with ethnical a...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2021/5544198 |
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| author | Yasir Ali Suleman Khan Yangchao Chen Nadia Farooqi Zia-Ul Islam Mehran Akhtar Aamir Aisha Aman Aftab Ali Shah Muhsin Jamal Fazal Jalil |
| author_facet | Yasir Ali Suleman Khan Yangchao Chen Nadia Farooqi Zia-Ul Islam Mehran Akhtar Aamir Aisha Aman Aftab Ali Shah Muhsin Jamal Fazal Jalil |
| author_sort | Yasir Ali |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is one of the complex diseases with the involvement of the genetic as well as environmental factors in its onset and severity. Different genome-wide association and candidate gene studies have shown the role of several genetic variants in multiple loci/genes with ethnical and geographical variations. This study was designed to detect the association of a single-nucleotide polymorphism (SNP) rs10865035 in the AFF3 gene with the genetic background of rheumatoid arthritis (RA) in the Pakistani cohort. A total of 703 individuals, including 409 RA patients and 294 healthy controls, were genotyped using TaqMan assay and Tri primer ARMS-PCR (amplification-refractory mutation system-polymerase chain reaction) methods. The association of rs10865035 with the RA was statistically determined using different models. Interestingly, besides the homozygous recessive model (G/G vs. A/G + A/A) (OR = 1.693(1.06–2.648); P = 0.025), all other models, which included the codominant (χ2 = 5.169; P = 0.075), homozygous dominant (A/A vs. G/G + A/G) (OR = 0.867 (0.636–1.187); P = 0.41), heterozygous (A/G vs. A/A + GG) (OR = 0.491 (0.667–1.215); P = 0.49), and additive model (OR = 0.826 (0.665–1.027); P = 0.08) showed insignificant distribution of the genotypes among the cases and controls. These findings suggest that the AFF3 gene (rs10865035) has no significant role in the onset of RA in the Pakistani population. |
| format | Article |
| id | doaj-art-8e55223486bb4e8181eafe90085068c7 |
| institution | Kabale University |
| issn | 0016-6723 1469-5073 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj-art-8e55223486bb4e8181eafe90085068c72025-02-03T00:59:41ZengWileyGenetics Research0016-67231469-50732021-01-01202110.1155/2021/55441985544198Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani CohortYasir Ali0Suleman Khan1Yangchao Chen2Nadia Farooqi3Zia-Ul Islam4Mehran Akhtar5Aamir6Aisha Aman7Aftab Ali Shah8Muhsin Jamal9Fazal Jalil10Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanLady Ready Hospital, MTI Peshawar, Peshawar, PakistanSchool of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, University of Malakand, Chakdara, PakistanDepartment of Microbiology, Abdul Wali Khan University Mardan, Mardan, PakistanDepartment of Biotechnology, Abdul Wali Khan University Mardan, Mardan, PakistanRheumatoid arthritis (RA) is one of the complex diseases with the involvement of the genetic as well as environmental factors in its onset and severity. Different genome-wide association and candidate gene studies have shown the role of several genetic variants in multiple loci/genes with ethnical and geographical variations. This study was designed to detect the association of a single-nucleotide polymorphism (SNP) rs10865035 in the AFF3 gene with the genetic background of rheumatoid arthritis (RA) in the Pakistani cohort. A total of 703 individuals, including 409 RA patients and 294 healthy controls, were genotyped using TaqMan assay and Tri primer ARMS-PCR (amplification-refractory mutation system-polymerase chain reaction) methods. The association of rs10865035 with the RA was statistically determined using different models. Interestingly, besides the homozygous recessive model (G/G vs. A/G + A/A) (OR = 1.693(1.06–2.648); P = 0.025), all other models, which included the codominant (χ2 = 5.169; P = 0.075), homozygous dominant (A/A vs. G/G + A/G) (OR = 0.867 (0.636–1.187); P = 0.41), heterozygous (A/G vs. A/A + GG) (OR = 0.491 (0.667–1.215); P = 0.49), and additive model (OR = 0.826 (0.665–1.027); P = 0.08) showed insignificant distribution of the genotypes among the cases and controls. These findings suggest that the AFF3 gene (rs10865035) has no significant role in the onset of RA in the Pakistani population.http://dx.doi.org/10.1155/2021/5544198 |
| spellingShingle | Yasir Ali Suleman Khan Yangchao Chen Nadia Farooqi Zia-Ul Islam Mehran Akhtar Aamir Aisha Aman Aftab Ali Shah Muhsin Jamal Fazal Jalil Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort Genetics Research |
| title | Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort |
| title_full | Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort |
| title_fullStr | Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort |
| title_full_unstemmed | Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort |
| title_short | Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort |
| title_sort | association of aff3 gene polymorphism rs10865035 with rheumatoid arthritis a population based case control study on a pakistani cohort |
| url | http://dx.doi.org/10.1155/2021/5544198 |
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