Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.)
The naleh fish (Barbonymus sp.) is an indigenous species found in Nagan Raya Regency, Aceh Province. The presence of Lactic Acid Bacteria (LAB) in the gastrointestinal tract of the fish may potentially act as probiotics, thus promoting the growth of the naleh fish. The objective of this research is...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03009.pdf |
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| author | Yulvizar Cut Yunita Dewi A Muchlizin Zainal Suhartono Suhartono |
| author_facet | Yulvizar Cut Yunita Dewi A Muchlizin Zainal Suhartono Suhartono |
| author_sort | Yulvizar Cut |
| collection | DOAJ |
| description | The naleh fish (Barbonymus sp.) is an indigenous species found in Nagan Raya Regency, Aceh Province. The presence of Lactic Acid Bacteria (LAB) in the gastrointestinal tract of the fish may potentially act as probiotics, thus promoting the growth of the naleh fish. The objective of this research is to isolate, characterise and select LAB through physiological testing. The research was conducted using a purposive sampling survey method and isolated the bacteria on selective media, namely Man, Rogosa, Sharpe (MRS) agar. The selection of physiological tests conducted included catalase tests, tests of the ability of the bacteria to survive at different temperatures, and tests of the effect of varying concentrations of NaCl. The research findings identified seven isolates with different colony characteristics of LAB. The morphology of the colonies was observed is punctiform (57.14%) and circular (42.85%). The colour is cream (14.28%) and white (85.71%). The edges were noted to be smooth (100%) and the elevation was observed to be flat (100%). The cell morphology is coccus - shaped (100%) and the bacteria are classified as Gram staining positive (100%). The growth of all LAB isolates from the gastrointestinal tract of the naleh fish is can survive by differences in temperature, NaCl concentration and catalase negative test. |
| format | Article |
| id | doaj-art-8e4871b751cf4255bd1ec20a5b0af6a7 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-8e4871b751cf4255bd1ec20a5b0af6a72025-02-05T10:43:33ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011560300910.1051/bioconf/202515603009bioconf_icfaes24_03009Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.)Yulvizar Cut0Yunita Dewi1A Muchlizin Zainal2Suhartono Suhartono3Graduate School of Mathematics and Applied Science, Universitas Syiah KualaDepartment of Agricultural Product Technology, Faculty of Agriculture, Universitas Syiah KualaDepartment of Marine Sciences, Faculty of Marine and Fisheries, Universitas Syiah KualaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah KualaThe naleh fish (Barbonymus sp.) is an indigenous species found in Nagan Raya Regency, Aceh Province. The presence of Lactic Acid Bacteria (LAB) in the gastrointestinal tract of the fish may potentially act as probiotics, thus promoting the growth of the naleh fish. The objective of this research is to isolate, characterise and select LAB through physiological testing. The research was conducted using a purposive sampling survey method and isolated the bacteria on selective media, namely Man, Rogosa, Sharpe (MRS) agar. The selection of physiological tests conducted included catalase tests, tests of the ability of the bacteria to survive at different temperatures, and tests of the effect of varying concentrations of NaCl. The research findings identified seven isolates with different colony characteristics of LAB. The morphology of the colonies was observed is punctiform (57.14%) and circular (42.85%). The colour is cream (14.28%) and white (85.71%). The edges were noted to be smooth (100%) and the elevation was observed to be flat (100%). The cell morphology is coccus - shaped (100%) and the bacteria are classified as Gram staining positive (100%). The growth of all LAB isolates from the gastrointestinal tract of the naleh fish is can survive by differences in temperature, NaCl concentration and catalase negative test.https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03009.pdf |
| spellingShingle | Yulvizar Cut Yunita Dewi A Muchlizin Zainal Suhartono Suhartono Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) BIO Web of Conferences |
| title | Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) |
| title_full | Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) |
| title_fullStr | Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) |
| title_full_unstemmed | Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) |
| title_short | Preliminary study on screening and selection of Lactic Acid Bacteria (LAB) from gastrointestinal tract of naleh fish (Barbonymus sp.) |
| title_sort | preliminary study on screening and selection of lactic acid bacteria lab from gastrointestinal tract of naleh fish barbonymus sp |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/07/bioconf_icfaes24_03009.pdf |
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