Is the Juice Worth the Squeeze? Evaluating Peptide Nucleic Acid Blockers for Reducing Plant DNA Contamination in 16S rRNA Gene Sequencing
Next-generation sequencing of microbial communities frequently results in off-target amplification of the host or environmental DNA, which can lead to substantial data loss. This challenge is particularly pronounced in plant-associated microbiome studies, in which commonly used 16S rRNA primers co-a...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2025-03-01
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| Series: | PhytoFrontiers |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-11-24-0124-SC |
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